2rc3
From Proteopedia
(New page: 200px<br /><applet load="2rc3" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rc3, resolution 1.60Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | We tested the general applicability of in situ proteolysis to form protein | + | We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Leo | + | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17982461 17982461] |
[[Category: Nitrosomonas europaea atcc 19718]] | [[Category: Nitrosomonas europaea atcc 19718]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dong, A.]] | [[Category: Dong, A.]] | ||
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
[[Category: Korniyenko, Y.]] | [[Category: Korniyenko, Y.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Savchenko, A.]] | [[Category: Savchenko, A.]] | ||
| - | [[Category: Walker, J | + | [[Category: Walker, J R.]] |
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
[[Category: Yakunin, A.]] | [[Category: Yakunin, A.]] | ||
| Line 35: | Line 35: | ||
[[Category: unknown function]] | [[Category: unknown function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:09 2008'' |
Revision as of 16:46, 21 February 2008
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Crystal structure of CBS domain, NE2398
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2RC3 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718 with and as ligands. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Thu Feb 21 18:46:09 2008
Categories: Nitrosomonas europaea atcc 19718 | Single protein | Dong, A. | Edwards, A M. | Joachimiak, A. | Korniyenko, Y. | MCSG, Midwest Center for Structural Genomics. | Savchenko, A. | Walker, J R. | Xu, X. | Yakunin, A. | Zheng, H. | BR | NAD | Br | Cbs domain | In situ proteolysis | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi-2 | Structural genomics | Unknown function
