2rcu
From Proteopedia
(New page: 200px<br /><applet load="2rcu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rcu, resolution 1.78Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The mitochondrial membrane-associated carnitine palmitoyltransferase | + | The mitochondrial membrane-associated carnitine palmitoyltransferase system is a validated target for the treatment of type 2 diabetes mellitus. To further facilitate structure-based drug discovery, we determined the crystal structure of rat CPT-2 (rCPT-2) in complex with the substrate analogue palmitoyl-aminocarnitine at 1.8A resolution. Biochemical analyses revealed a strong effect of this compound on rCPT-2 activity and stability. Using a computational approach we examined the membrane association of rCPT-2. The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Chomienne, O.]] | [[Category: Chomienne, O.]] | ||
[[Category: Hennig, M.]] | [[Category: Hennig, M.]] | ||
| - | [[Category: Rufer, A | + | [[Category: Rufer, A C.]] |
[[Category: Thoma, R.]] | [[Category: Thoma, R.]] | ||
[[Category: BOG]] | [[Category: BOG]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:15 2008'' |
Revision as of 16:46, 21 February 2008
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Crystal structure of rat carnitine palmitoyltransferase 2 in complex with r-3-(hexadecanoylamino)-4-(trimethylazaniumyl)butanoate
Overview
The mitochondrial membrane-associated carnitine palmitoyltransferase system is a validated target for the treatment of type 2 diabetes mellitus. To further facilitate structure-based drug discovery, we determined the crystal structure of rat CPT-2 (rCPT-2) in complex with the substrate analogue palmitoyl-aminocarnitine at 1.8A resolution. Biochemical analyses revealed a strong effect of this compound on rCPT-2 activity and stability. Using a computational approach we examined the membrane association of rCPT-2. The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues.
About this Structure
2RCU is a Single protein structure of sequence from Rattus norvegicus with and as ligands. Active as Carnitine O-palmitoyltransferase, with EC number 2.3.1.21 Full crystallographic information is available from OCA.
Reference
Carnitine palmitoyltransferase 2: analysis of membrane association and complex structure with a substrate analog., Rufer AC, Lomize A, Benz J, Chomienne O, Thoma R, Hennig M, FEBS Lett. 2007 Jul 10;581(17):3247-52. Epub 2007 Jun 8. PMID:17585909
Page seeded by OCA on Thu Feb 21 18:46:15 2008
Categories: Carnitine O-palmitoyltransferase | Rattus norvegicus | Single protein | Benz, J. | Chomienne, O. | Hennig, M. | Rufer, A C. | Thoma, R. | BOG | BUJ | Acetylation | Acyltransferase | Fatty acid metabolism | Inner membrane | Lipid metabolism | Membrane | Mitochondrial protein | Mitochondrion | Transferase | Transferase 04-mai-06 r | Transit peptide | Transport
