2rll

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==Overview==
==Overview==
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The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and, facilitates HIV-1 entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on, gp120. We applied nuclear magnetic resonance and crystallographic, techniques to analyze the structure of the CCR5 N terminus and that of the, tyrosine-sulfated antibody 412d in complex with gp120 and CD4. The, conformations of tyrosine-sulfated regions of CCR5 (alpha-helix) and 412d, (extended loop) are surprisingly different. Nonetheless, a critical, sulfotyrosine on CCR5 and on 412d induces similar structural, rearrangements in gp120. These results now provide a framework for, understanding HIV-1 interactions with the CCR5 N terminus during viral, entry and define a conserved site on gp120, whose recognition of, sulfotyrosine engenders posttranslational mimicry by the immune system.
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The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates HIV-1 entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on gp120. We applied nuclear magnetic resonance and crystallographic techniques to analyze the structure of the CCR5 N terminus and that of the tyrosine-sulfated antibody 412d in complex with gp120 and CD4. The conformations of tyrosine-sulfated regions of CCR5 (alpha-helix) and 412d (extended loop) are surprisingly different. Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar structural rearrangements in gp120. These results now provide a framework for understanding HIV-1 interactions with the CCR5 N terminus during viral entry and define a conserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the immune system.
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==Disease==
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Known diseases associated with this structure: HIV infection, susceptibility/resistance to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601373 601373]], West nile virus, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601373 601373]]
==About this Structure==
==About this Structure==
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Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4., Huang CC, Lam SN, Acharya P, Tang M, Xiang SH, Hussan SS, Stanfield RL, Robinson J, Sodroski J, Wilson IA, Wyatt R, Bewley CA, Kwong PD, Science. 2007 Sep 28;317(5846):1930-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17901336 17901336]
Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4., Huang CC, Lam SN, Acharya P, Tang M, Xiang SH, Hussan SS, Stanfield RL, Robinson J, Sodroski J, Wilson IA, Wyatt R, Bewley CA, Kwong PD, Science. 2007 Sep 28;317(5846):1930-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17901336 17901336]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Bewley, C.A.]]
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[[Category: Bewley, C A.]]
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[[Category: Lam, S.N.]]
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[[Category: Lam, S N.]]
[[Category: g-protein coupled receptor]]
[[Category: g-protein coupled receptor]]
[[Category: glycoprotein]]
[[Category: glycoprotein]]
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[[Category: transmembrane]]
[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:51:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:48:18 2008''

Revision as of 16:48, 21 February 2008


2rll

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CCR5 Nt(7-15)

Contents

Overview

The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates HIV-1 entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on gp120. We applied nuclear magnetic resonance and crystallographic techniques to analyze the structure of the CCR5 N terminus and that of the tyrosine-sulfated antibody 412d in complex with gp120 and CD4. The conformations of tyrosine-sulfated regions of CCR5 (alpha-helix) and 412d (extended loop) are surprisingly different. Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar structural rearrangements in gp120. These results now provide a framework for understanding HIV-1 interactions with the CCR5 N terminus during viral entry and define a conserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the immune system.

Disease

Known diseases associated with this structure: HIV infection, susceptibility/resistance to OMIM:[601373], West nile virus, susceptibility to OMIM:[601373]

About this Structure

2RLL is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4., Huang CC, Lam SN, Acharya P, Tang M, Xiang SH, Hussan SS, Stanfield RL, Robinson J, Sodroski J, Wilson IA, Wyatt R, Bewley CA, Kwong PD, Science. 2007 Sep 28;317(5846):1930-4. PMID:17901336

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