2tps
From Proteopedia
(New page: 200px<br /><applet load="2tps" size="450" color="white" frame="true" align="right" spinBox="true" caption="2tps, resolution 1.25Å" /> '''THIAMIN PHOSPHATE SY...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2tps.gif|left|200px]]<br /><applet load="2tps" size=" | + | [[Image:2tps.gif|left|200px]]<br /><applet load="2tps" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2tps, resolution 1.25Å" /> | caption="2tps, resolution 1.25Å" /> | ||
'''THIAMIN PHOSPHATE SYNTHASE'''<br /> | '''THIAMIN PHOSPHATE SYNTHASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is | + | The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is described. The first gene in the operon is homologous to transcription activators in the lysR family. The second and third genes (thiK and thiC) have been subcloned and overexpressed in Escherichia coli. ThiK (30 kDa) catalyzes the phosphorylation of 4-methyl-5-(beta-hydroxyethyl)thiazole. ThiC (27 kDa) catalyzes the substitution of the pyrophosphate of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate. Transcription of the thiC operon is not regulated by thiamine or 2-methyl-4-amino-5-hydroxymethylpyrimidine and is only slightly repressed by 4-methyl-5-(beta-hydroxyethyl)thiazole. |
==About this Structure== | ==About this Structure== | ||
| - | 2TPS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MG, TPS and POP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] Full crystallographic information is available from [http:// | + | 2TPS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=TPS:'>TPS</scene> and <scene name='pdbligand=POP:'>POP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TPS OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thiamine-phosphate diphosphorylase]] | [[Category: Thiamine-phosphate diphosphorylase]] | ||
| - | [[Category: Begley, T | + | [[Category: Begley, T P.]] |
| - | [[Category: Chiu, H | + | [[Category: Chiu, H J.]] |
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
| - | [[Category: Reddick, J | + | [[Category: Reddick, J J.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: POP]] | [[Category: POP]] | ||
| Line 24: | Line 24: | ||
[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:57 2008'' |
Revision as of 16:50, 21 February 2008
|
THIAMIN PHOSPHATE SYNTHASE
Overview
The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is described. The first gene in the operon is homologous to transcription activators in the lysR family. The second and third genes (thiK and thiC) have been subcloned and overexpressed in Escherichia coli. ThiK (30 kDa) catalyzes the phosphorylation of 4-methyl-5-(beta-hydroxyethyl)thiazole. ThiC (27 kDa) catalyzes the substitution of the pyrophosphate of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate. Transcription of the thiC operon is not regulated by thiamine or 2-methyl-4-amino-5-hydroxymethylpyrimidine and is only slightly repressed by 4-methyl-5-(beta-hydroxyethyl)thiazole.
About this Structure
2TPS is a Single protein structure of sequence from Bacillus subtilis with , and as ligands. Active as Thiamine-phosphate diphosphorylase, with EC number 2.5.1.3 Full crystallographic information is available from OCA.
Reference
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis., Zhang Y, Taylor SV, Chiu HJ, Begley TP, J Bacteriol. 1997 May;179(9):3030-5. PMID:9139923
Page seeded by OCA on Thu Feb 21 18:49:57 2008
