2ush
From Proteopedia
(New page: 200px<br /><applet load="2ush" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ush, resolution 2.22Å" /> '''5'-NUCLEOTIDASE FROM...) |
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| - | [[Image:2ush.jpg|left|200px]]<br /><applet load="2ush" size=" | + | [[Image:2ush.jpg|left|200px]]<br /><applet load="2ush" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ush, resolution 2.22Å" /> | caption="2ush, resolution 2.22Å" /> | ||
'''5'-NUCLEOTIDASE FROM E. COLI'''<br /> | '''5'-NUCLEOTIDASE FROM E. COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known | + | The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known as UDP-sugar hydrolase, has been determined at 1.7 A resolution. Two zinc ions are present in the active site, which is located in a cleft between two domains. The dimetal center and a catalytic Asp-His dyad are the main players in the catalytic mechanism. Structure-based sequence comparisons show that the structure also provides a model for animal 5'-NTs, which together with other ectonucleotidases terminate the action of nucleotides as extracellular signaling substances in the nervous system. |
==About this Structure== | ==About this Structure== | ||
| - | 2USH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and WO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] Full crystallographic information is available from [http:// | + | 2USH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=WO4:'>WO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2USH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: WO4]] | [[Category: WO4]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: 5'-nucleotidase]] | ||
[[Category: hydrolase (phosphoric monoester)]] | [[Category: hydrolase (phosphoric monoester)]] | ||
[[Category: periplasmic protein]] | [[Category: periplasmic protein]] | ||
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[[Category: udp-sugar hydrolase]] | [[Category: udp-sugar hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:50:15 2008'' |
Revision as of 16:50, 21 February 2008
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5'-NUCLEOTIDASE FROM E. COLI
Overview
The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known as UDP-sugar hydrolase, has been determined at 1.7 A resolution. Two zinc ions are present in the active site, which is located in a cleft between two domains. The dimetal center and a catalytic Asp-His dyad are the main players in the catalytic mechanism. Structure-based sequence comparisons show that the structure also provides a model for animal 5'-NTs, which together with other ectonucleotidases terminate the action of nucleotides as extracellular signaling substances in the nervous system.
About this Structure
2USH is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as 5'-nucleotidase, with EC number 3.1.3.5 Full crystallographic information is available from OCA.
Reference
X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:10331872
Page seeded by OCA on Thu Feb 21 18:50:15 2008
