2ux8
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Gellan gum is a widely used commercial material, available in many | + | Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the reversible conversion of glucose-1-phosphate and UTP into UDP-glucose and pyrophosphate, which is a key step in the biosynthetic pathway of gellan gums. Here we present the X-ray crystal structure of the glucose-1-phosphate uridylyltransferase from S. elodea. The S. elodea enzyme shares strong monomeric similarity with glucose-1-phosphate thymidylyltransferase, several structures of which are known, although the quaternary structures of the active enzymes are rather different. A detailed comparison between S. elodea glucose-1-phosphate uridylyltransferase and available thymidylyltransferases is described and shows remarkable structural similarities, despite the low sequence identities between the two divergent groups of proteins. |
==About this Structure== | ==About this Structure== | ||
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[[Category: UTP--glucose-1-phosphate uridylyltransferase]] | [[Category: UTP--glucose-1-phosphate uridylyltransferase]] | ||
[[Category: Aragao, D.]] | [[Category: Aragao, D.]] | ||
| - | [[Category: Fialho, A | + | [[Category: Fialho, A M.]] |
[[Category: Frazao, C.]] | [[Category: Frazao, C.]] | ||
| - | [[Category: Marques, A | + | [[Category: Marques, A R.]] |
| - | [[Category: Mitchell, E | + | [[Category: Mitchell, E P.]] |
[[Category: Sa-Correia, I.]] | [[Category: Sa-Correia, I.]] | ||
[[Category: G1P]] | [[Category: G1P]] | ||
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[[Category: ugpg]] | [[Category: ugpg]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:51:15 2008'' |
Revision as of 16:51, 21 February 2008
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CRYSTAL STRUCTURE OF SPHINGOMONAS ELODEA ATCC 31461 GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE IN COMPLEX WITH GLUCOSE-1-PHOSPHATE.
Overview
Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the reversible conversion of glucose-1-phosphate and UTP into UDP-glucose and pyrophosphate, which is a key step in the biosynthetic pathway of gellan gums. Here we present the X-ray crystal structure of the glucose-1-phosphate uridylyltransferase from S. elodea. The S. elodea enzyme shares strong monomeric similarity with glucose-1-phosphate thymidylyltransferase, several structures of which are known, although the quaternary structures of the active enzymes are rather different. A detailed comparison between S. elodea glucose-1-phosphate uridylyltransferase and available thymidylyltransferases is described and shows remarkable structural similarities, despite the low sequence identities between the two divergent groups of proteins.
About this Structure
2UX8 is a Single protein structure of sequence from Sphingomonas elodea with as ligand. Active as UTP--glucose-1-phosphate uridylyltransferase, with EC number 2.7.7.9 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase members., Aragao D, Fialho AM, Marques AR, Mitchell EP, Sa-Correia I, Frazao C, J Bacteriol. 2007 Jun;189(12):4520-8. Epub 2007 Apr 13. PMID:17434970
Page seeded by OCA on Thu Feb 21 18:51:15 2008
Categories: Single protein | Sphingomonas elodea | UTP--glucose-1-phosphate uridylyltransferase | Aragao, D. | Fialho, A M. | Frazao, C. | Marques, A R. | Mitchell, E P. | Sa-Correia, I. | G1P | Galu pyrophosphorylase | Glucose-1-phosphate uridylyltransferase | Nucleotidyltransferase | Transferase | Ugpg
