Purine nucleoside phosphorylase (PNP) cleaves nucleosides by phosphorylating ribose to produce ribose-1-phosphate. PNP metabolizes adenosine, inosine and guanosine. PNP metabolizes the waste molecules deoxyinosine and deoxyguanosine which are formed when DNA breaks down. PNP deficiency results in severe combined immunodeficiency.
3D structures of purine nucleoside phosphorylase
1ula, 1m73 – hPNP – human
1pbn – bPNP – bovine
1odk, 1odl – TtPNP – Thermus thermophilus
1z33 – TvPNP – Trichomonas vaginalis
1tcv, 1td1 – SmPNP – Schistosoma masoni
2b94 – PNP – Plasmodium knowlesi
4mar – MrPNP – Meiothermus ruber
PNP binary complex with phosphate
1a9o, 3pnp, 4pnp – bPNP + phosphate
1qe5 – CePNP + phosphate - Cellulomonas
1tcu – SmPNP + phosphate
3khs – PNP + phosphate – grouper iridovirus
4gka – hPNP (mutant) + phosphate
4m3n – MrPNP + phosphate
4m7w – PNP + phosphate – Leptotrichia buccalis
PNP complex with guanine and derivatives
1ulb, 1v2h – hPNP + guanine
4ece – hPNP (mutant) + guanine
1rfg – hPNP + guanosine
1yry, 3iny – hPNP + guanine derivative
1v48, 2ai1, 2ai3, 3fuc – bPNP + guanine derivative
3e9z – SmPNP + guanine derivative
3iex – SmPNP + guanosine
1odj – TtPNP + guanosine
1vmk – PNP + guanine – Thermotoga maritima
3iom – MtPNP + guanine derivative - Mycobacterium tuberculosis
1b8n – bPNP + guanine derivative + phosphate
1c3x – CePNP + guanine derivative + phosphate
1pwy, 1v41, 1v45 – hPNP + antiviral drug
PNP complex with inosine and derivatives
1rct – hPNP + inosine
1a9q, 1a9s – bPNP + inosine
3faz – SmPNP + inosine
2ai2 – bPNP + inosine derivative
1z38 – TvPNP + inosine
1z39 – TvPNP + deoxyinosine
1a9t, 1a9p – bPNP + deazainosine + phosphate
1v3q – hPNP + anti HIV drug
PNP complex with xanthine and derivatives
1pf7, 1rr6, 1rsz, 1rt9, 3k8o, 3k8q – hPNP + transition state analog
2on6 – hPNP (mutant) + transition state analog
1vfn, 1a9r – bPNP + hypoxanthine
3fnq – SmPNP + hypoxanthine
3scz – MtPNP + hypoxanthine
2a0w, 2a0x, 2a0y, 2oc4, 2oc9 – hPNP (mutant) + hypoxanthine derivative
3odg – PNP + xanthine – Yersinia pseudotuberculosis
1b8o, 1g2o – bPNP + hypoxanthine derivative + phosphate
3bgs – bPNP + transition state analog + phosphate
2q7o, 3phb – hPNP + transition state analog + phosphate
4ear, 4eb8 – hPNP (mutant) + transition state analog + phosphate
3mb8 – PNP + transition state analog + phosphate – Toxoplasma gondii
1n3i – MtPNP + deazahypoxanthine + phosphate
1i80 – MtPNP + iminoribitol + deazahypoxanthine + phosphate
PNP complex with adenine and derivatives
1odi – TtPNP + adenosine
1z34, 1z35 – TvPNP + adenosine derivative
4ns1 – PgPNP + adenosine derivative – Porphyromonas gingivalis
4nsn – PgPNP + adenine
3ggs – hPNP (mutant) + adenosine derivative
1z37 – TvPNP + adenosine
3e9r – SmPNP + adenine
3f8w – SmPNP + adenosine
4m1e – PNP + adenine – Planctomyces limnophilus
4lna – PNP + adenine + phosphate – Spirosoma linguale
PNP complex with purine and derivatives
1lv8, 1lvu, 2qpl – bPNP + purine derivative
3gb9 – hPNP (mutant) + purine derivative
1fxu – bPNP + purine derivative + phosphate
1z36 – TvPNP + antibiotic
3djf – SmPNP + inhibitor
Other PNP complexes
3d1v – hPNP + mercapto-quinazolinone
3e0q – SmPNP + pyrimidine derivative
3fb1 – SmPNP + ribose-1-phosphate
