Penicillin acylase
From Proteopedia
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| - | {{STRUCTURE_1fxv| PDB=1fxv | SIZE=400| SCENE= |right|CAPTION=''E. coli'' penicillin acylase small α subunit (grey) and large β subunit (green) complex with penicillin and Ca+2 ion, [[1fxv]] }} | + | {{STRUCTURE_1fxv| PDB=1fxv | SIZE=400| SCENE= |right|CAPTION=''E. coli'' penicillin acylase small α subunit (grey) and large β subunit (green) complex with penicillin and Ca+2 ion (green), [[1fxv]] }} |
'''Penicillin acylase''' (PAH) catalyzes the conversion of penicillin to 6-amino-penicillanate and phenylacetate (PA). PAH contains 2 non-identical subunits. The larger β subunit contains a phenylmethylsulfonyl residue which is required for enzymatic activity. PAH participates in penicillin and cephalosporin biosynthesis. PAH is used commercially for production of semi-synthetic penicillins. | '''Penicillin acylase''' (PAH) catalyzes the conversion of penicillin to 6-amino-penicillanate and phenylacetate (PA). PAH contains 2 non-identical subunits. The larger β subunit contains a phenylmethylsulfonyl residue which is required for enzymatic activity. PAH participates in penicillin and cephalosporin biosynthesis. PAH is used commercially for production of semi-synthetic penicillins. | ||
Revision as of 11:44, 29 December 2013
Penicillin acylase (PAH) catalyzes the conversion of penicillin to 6-amino-penicillanate and phenylacetate (PA). PAH contains 2 non-identical subunits. The larger β subunit contains a phenylmethylsulfonyl residue which is required for enzymatic activity. PAH participates in penicillin and cephalosporin biosynthesis. PAH is used commercially for production of semi-synthetic penicillins.
3D structures of penicillin acylase
1pnk, 1pnl, 1pnm, 1gk9 – EcPAH – Escherichia coli
1gkf, 1h2g, 1jx9 - EcPAH α + β (mutant) subunits
1e3a – EcPAH precursor α + β (mutant) subunits
3pva, 2pva – PAH – Lysinibacillus sphaericus
2iwm - BsPAH (mutant) – Bacillus sphaericus
3mji - BsPAH
2oqc - PAH – Bacillus subtilis
3k3w, 3ml0 - PAH precursor – Alcaligenes faecalis
Penicillin acylase complex
1fxh, 1k5q - EcPAH α + β (mutant) subunits + PA
1fxv, 1gm7 - EcPAH α + β (mutant) subunits + penicillin
1gm9 - EcPAH α + β (mutant) subunits + penicillin derivative
1gm8, 1k5s, 1kec - EcPAH α + β (mutant) subunits + PA derivative
1ai4, 1ai5, 1ai6, 1ajn, 1ajp, 1ajq, 1k7d – EcPAH + PA derivative
1ai7 – EcPAH + phenol
2z71 - BsPAH (mutant) + penicillin
