2bhz

From Proteopedia

Revision as of 14:33, 30 October 2007

CRYSTAL STRUCTURE OF DEINOCOCCUS RADIODURANS MALTOOLIGOSYLTREHALOSE TREHALOHYDROLASE IN COMPLEX WITH MALTOSE

Overview

Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranose) is a, non-reducing diglucoside found in various organisms that serves as a, carbohydrate reserve and as an agent that protects against a variety of, physical and chemical stresses. Deinococcus radiodurans possesses an, alternative biosynthesis pathway for the synthesis of trehalose from, maltooligosaccharides. This reaction is mediated by two enzymes:, maltooligosyltrehalose synthase (MTSase) and maltooligosyltrehalose, trehalohydrolase (MTHase). Here, we present the 1.1A resolution crystal, structure of MTHase. It consists of three major domains: two beta-sheet, domains and a conserved glycosidase (beta/alpha)8 barrel catalytic domain., Three subdomains consisting of short insertions were identified within the, catalytic ... [(full description)]

About this Structure

2BHZ is a [Single protein] structure of sequence from [Deinococcus radiodurans] with MAL, MG, BME and TRS as [ligands]. Active as [Alpha-amylase], with EC number [3.2.1.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of maltooligosyltrehalose trehalohydrolase from Deinococcus radiodurans in complex with disaccharides., Timmins J, Leiros HK, Leonard G, Leiros I, McSweeney S, J Mol Biol. 2005 Apr 15;347(5):949-63. PMID:15784255

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