2v79
From Proteopedia
(New page: 200px<br /><applet load="2v79" size="350" color="white" frame="true" align="right" spinBox="true" caption="2v79, resolution 2.00Å" /> '''CRYSTAL STRUCTURE OF...) |
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==Overview== | ==Overview== | ||
| - | The DnaD protein is an essential component of the chromosome-replication | + | The DnaD protein is an essential component of the chromosome-replication machinery of the Gram-positive bacterium Bacillus subtilis and is part of the primosomal cascade that ultimately loads the replicative ring helicase DnaC onto DNA. Moreover, DnaD is a global regulator of DNA architecture, as it forms higher order nucleoprotein structures in order to open supercoiled DNA. Here, the crystallization and preliminary X-ray diffraction analysis of the two domains of DnaD from B. subtilis are reported. Crystals of the N-terminal domain are trigonal, with either P3(1)21 or P3(2)21 space-group symmetry, and diffracted X-rays to 2.0 A resolution; crystals of the C-terminal domain are hexagonal, with space group P6(1) or P6(5), and diffracted X-rays to 2.9 A resolution in-house. Determination of the structure of the DnaD domains will provide insight into how remodelling of the nucleoid is associated with priming of replication in the model Gram-positive organism B. subtilis. |
==About this Structure== | ==About this Structure== | ||
| - | 2V79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Na Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Na Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Na Binding Site For Chain B'>AC3</scene>, <scene name='pdbsite=AC4:Cl Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Cl Binding Site For Chain A'>AC5</scene>, <scene name='pdbsite=AC6:Cl Binding Site For Chain B'>AC6</scene>, <scene name='pdbsite=AC7:Cl Binding Site For Chain B'>AC7</scene>, <scene name='pdbsite=AC8:Cl Binding Site For Chain B'>AC8</scene>, <scene name='pdbsite=AC9:Cl Binding Site For Chain B'>AC9</scene>, <scene name='pdbsite=BC1:Cl Binding Site For Chain A'>BC1</scene>, <scene name='pdbsite=BC2:Cl Binding Site For Chain A'>BC2</scene>, <scene name='pdbsite=BC3:Cl Binding Site For Chain A'>BC3</scene>, <scene name='pdbsite=BC4:Cl Binding Site For Chain B'>BC4</scene> and <scene name='pdbsite=BC5:Cl Binding Site For Chain B'>BC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V79 OCA]. | + | 2V79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Na+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Na+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Cl+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Cl+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Cl+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Cl+Binding+Site+For+Chain+B'>AC7</scene>, <scene name='pdbsite=AC8:Cl+Binding+Site+For+Chain+B'>AC8</scene>, <scene name='pdbsite=AC9:Cl+Binding+Site+For+Chain+B'>AC9</scene>, <scene name='pdbsite=BC1:Cl+Binding+Site+For+Chain+A'>BC1</scene>, <scene name='pdbsite=BC2:Cl+Binding+Site+For+Chain+A'>BC2</scene>, <scene name='pdbsite=BC3:Cl+Binding+Site+For+Chain+A'>BC3</scene>, <scene name='pdbsite=BC4:Cl+Binding+Site+For+Chain+B'>BC4</scene> and <scene name='pdbsite=BC5:Cl+Binding+Site+For+Chain+B'>BC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V79 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: primosome]] | [[Category: primosome]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:53:39 2008'' |
Revision as of 16:53, 21 February 2008
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CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF DNAD FROM BACILLUS SUBTILIS
Overview
The DnaD protein is an essential component of the chromosome-replication machinery of the Gram-positive bacterium Bacillus subtilis and is part of the primosomal cascade that ultimately loads the replicative ring helicase DnaC onto DNA. Moreover, DnaD is a global regulator of DNA architecture, as it forms higher order nucleoprotein structures in order to open supercoiled DNA. Here, the crystallization and preliminary X-ray diffraction analysis of the two domains of DnaD from B. subtilis are reported. Crystals of the N-terminal domain are trigonal, with either P3(1)21 or P3(2)21 space-group symmetry, and diffracted X-rays to 2.0 A resolution; crystals of the C-terminal domain are hexagonal, with space group P6(1) or P6(5), and diffracted X-rays to 2.9 A resolution in-house. Determination of the structure of the DnaD domains will provide insight into how remodelling of the nucleoid is associated with priming of replication in the model Gram-positive organism B. subtilis.
About this Structure
2V79 is a Single protein structure of sequence from Bacillus subtilis with and as ligands. Known structural/functional Sites: , , , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray diffraction analysis of the DNA-remodelling protein DnaD from Bacillus subtilis., Schneider S, Carneiro MJ, Ioannou C, Soultanas P, Paoli M, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt, 2):110-3. Epub 2007 Jan 17. PMID:17277452
Page seeded by OCA on Thu Feb 21 18:53:39 2008
