2v8o
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Murray Valley encephalitis virus (MVEV), a mosquito-borne flavivirus | + | Murray Valley encephalitis virus (MVEV), a mosquito-borne flavivirus endemic to Australia, is closely related to Japanese encephalitis virus and West Nile virus. Nonstructural protein 3 (NS3) is a multifunctional enzyme with serine protease and DEXH/D-box helicase domains, whose activity is central to flavivirus replication and is therefore a possible target for anti-flaviviral compounds. Cloning, purification, and crystal structure determination to 1.9 Angstrom resolution of the NS3 helicase of MVEV and characterization of its enzymatic activity is reported. Comparison with the structures of helicases from related viruses supports a possible mechanism of ATP hydrolysis-driven strand separation. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 | + | Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution., Mancini EJ, Assenberg R, Verma A, Walter TS, Tuma R, Grimes JM, Owens RJ, Stuart DI, Protein Sci. 2007 Oct;16(10):2294-300. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17893366 17893366] |
[[Category: Flavivirin]] | [[Category: Flavivirin]] | ||
[[Category: Murray valley encephalitis virus]] | [[Category: Murray valley encephalitis virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Assenberg, R.]] | [[Category: Assenberg, R.]] | ||
| - | [[Category: Grimes, J | + | [[Category: Grimes, J M.]] |
| - | [[Category: Mancini, E | + | [[Category: Mancini, E J.]] |
| - | [[Category: Owens, R | + | [[Category: Owens, R J.]] |
| - | [[Category: Stuart, D | + | [[Category: Stuart, D I.]] |
[[Category: Tuma, R.]] | [[Category: Tuma, R.]] | ||
[[Category: Verma, A.]] | [[Category: Verma, A.]] | ||
| - | [[Category: Walter, T | + | [[Category: Walter, T S.]] |
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: capsid protein]] | [[Category: capsid protein]] | ||
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[[Category: virion]] | [[Category: virion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:54:04 2008'' |
Revision as of 16:54, 21 February 2008
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STRUCTURE OF THE MURRAY VALLEY ENCEPHALITIS VIRUS RNA HELICASE TO 1.9A RESOLUTION
Overview
Murray Valley encephalitis virus (MVEV), a mosquito-borne flavivirus endemic to Australia, is closely related to Japanese encephalitis virus and West Nile virus. Nonstructural protein 3 (NS3) is a multifunctional enzyme with serine protease and DEXH/D-box helicase domains, whose activity is central to flavivirus replication and is therefore a possible target for anti-flaviviral compounds. Cloning, purification, and crystal structure determination to 1.9 Angstrom resolution of the NS3 helicase of MVEV and characterization of its enzymatic activity is reported. Comparison with the structures of helicases from related viruses supports a possible mechanism of ATP hydrolysis-driven strand separation.
About this Structure
2V8O is a Single protein structure of sequence from Murray valley encephalitis virus. Active as Flavivirin, with EC number 3.4.21.91 Full crystallographic information is available from OCA.
Reference
Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution., Mancini EJ, Assenberg R, Verma A, Walter TS, Tuma R, Grimes JM, Owens RJ, Stuart DI, Protein Sci. 2007 Oct;16(10):2294-300. PMID:17893366
Page seeded by OCA on Thu Feb 21 18:54:04 2008
Categories: Flavivirin | Murray valley encephalitis virus | Single protein | Assenberg, R. | Grimes, J M. | Mancini, E J. | Owens, R J. | Stuart, D I. | Tuma, R. | Verma, A. | Walter, T S. | Atp-binding | Capsid protein | Cleavage on pair of basic residues | Core protein | Envelope protein | Flaviviridae | Glycoprotein | Helicase | Helicases | Hydrolase | Membrane | Nucleotide-binding | Nucleotidyltransferase | Rna replication | Rna-directed rna polymerase | Transferase | Transmembrane | Viral enzymes | Virion
