2v9y
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | We tested the general applicability of in situ proteolysis to form protein | + | We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Leo | + | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17982461 17982461] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Phosphoribosylformylglycinamidine cyclo-ligase]] | [[Category: Phosphoribosylformylglycinamidine cyclo-ligase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Berg, S | + | [[Category: Berg, S Van Den.]] |
[[Category: Berglund, H.]] | [[Category: Berglund, H.]] | ||
[[Category: Busam, R.]] | [[Category: Busam, R.]] | ||
[[Category: Collins, R.]] | [[Category: Collins, R.]] | ||
| - | [[Category: Consortium, Structural | + | [[Category: Consortium, Structural Genomics.]] |
| - | [[Category: Dahlgren, L | + | [[Category: Dahlgren, L G.]] |
[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
[[Category: Flodin, S.]] | [[Category: Flodin, S.]] | ||
[[Category: Flores, A.]] | [[Category: Flores, A.]] | ||
[[Category: Graslund, S.]] | [[Category: Graslund, S.]] | ||
| - | [[Category: Hallberg, B | + | [[Category: Hallberg, B M.]] |
[[Category: Hammarstrom, M.]] | [[Category: Hammarstrom, M.]] | ||
| - | [[Category: Herman, M | + | [[Category: Herman, M D.]] |
[[Category: Holmberg-Schiavone, L.]] | [[Category: Holmberg-Schiavone, L.]] | ||
[[Category: Johansson, I.]] | [[Category: Johansson, I.]] | ||
| Line 41: | Line 41: | ||
[[Category: Stenmark, P.]] | [[Category: Stenmark, P.]] | ||
[[Category: Sundstrom, M.]] | [[Category: Sundstrom, M.]] | ||
| - | [[Category: Thorsell, A | + | [[Category: Thorsell, A G.]] |
[[Category: Tresaugues, L.]] | [[Category: Tresaugues, L.]] | ||
[[Category: Weigelt, J.]] | [[Category: Weigelt, J.]] | ||
| Line 63: | Line 63: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:54:25 2008'' |
Revision as of 16:54, 21 February 2008
|
HUMAN AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2V9Y is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Phosphoribosylformylglycinamidine cyclo-ligase, with EC number 6.3.3.1 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Thu Feb 21 18:54:25 2008
Categories: Homo sapiens | Phosphoribosylformylglycinamidine cyclo-ligase | Single protein | Arrowsmith, C H. | Berg, S Van Den. | Berglund, H. | Busam, R. | Collins, R. | Consortium, Structural Genomics. | Dahlgren, L G. | Edwards, A. | Flodin, S. | Flores, A. | Graslund, S. | Hallberg, B M. | Hammarstrom, M. | Herman, M D. | Holmberg-Schiavone, L. | Johansson, I. | Kallas, A. | Karlberg, T. | Kotenyova, T. | Lehtio, L. | Moche, M. | Nordlund, P. | Nyman, T. | Persson, C. | Sagemark, J. | Stenmark, P. | Sundstrom, M. | Thorsell, A G. | Tresaugues, L. | Weigelt, J. | Welin, M. | SO4 | Airs | Alternative splicing | Aminoimidazole ribonucleotide synthetase | Atp-binding | Gart | Ligase | Multifunctional enzyme | Nucleotide-binding | Phosphorylation | Polymorphism | Purine biosynthesis | Purine metabolism | Sgc | Structural genomics | Structural genomics consortium | Transferase
