Sandbox Reserved 827

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This Sandbox is Reserved from 06/12/2018, through 30/06/2019 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1480 through Sandbox Reserved 1543.

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TBK1 stands for Tank Binding Kinase 1. This enzyme is a cytoplasmic serine-threonine kinase, coded by EC=2.7.11.1. It catalyzes the transfer of a phosphate group from an ATP onto a protein, in order to form a phosphoprotein and an ADP. Its primary sequence is 729 residues long. This homodimere is involved in several signalization pathways such as the inhibition of apoptosis, inflammatory response. Its substrates are NF-κ-B, various IRFs (interferon regulatory factors), DDX3X. It is involved in several complexes depending on the cell type and the stimuli.

==Your Heading Here (maybe something like 'Structure')==

= Overall structure =

Protomer

Kinase domain : ( ) from amino acid 9 to amino acid 310: the active site is at the interface of the N- and C-terminal lobes. Inactive conformation: the C-helix and key residue Glu55 displaced from the active site. Active conformation: a rotation of the C-helix allows a key salt-bridge interaction between conserved glutamic acid in the C-helix and an active-site lysine residue. The DFG (Asp-Phe-Gly) motif and Ser172 are involved in the regulation of the kinase activity.

Ubiquitin-like domain : ( ) from amino acid 309 to amino acid 385: it contains five β strands which form a hydrophobic interface.

: between amino acid 408 and amino acid 651

Coiled coil : between amino acid 408 and amino acid 651

ATP binding domain : from amino acid 15 to amino acid 23.

The KD and the ULD interact with each other: the ULD with the C-lobe of the KD. There is a hydrogen bond between Tyr325 in the ULD and Glu109 in the KD. Moreover Lys323 in the ULD makes favourable electrostatic interactions with Glu109-KD.

Dimer

ULD and KD also contribute to dimerization thanks to several interactions with the SDD of the opposite subunit in the homodimer. There are several hydrogen bonds between the KD (N- and C-lobes) and the SDD of the opposite subunit: a salt bridge is formed between Asp33 in the N-lobe and Lys589 in the SDD and the strands the β7-β8 in the C-love interact with the SDD. A “EGR” sequence (residues 355–357) in the ULD interacts with the SDD: Glu355-ULD interacts with Arg444-SDD (salt bridge) and Trp445-SDD (hydrogen bonds).

Possible residue modifications

Several residues of TBK1 can be modified, by phosphorylation or polyubiquitination.

Phosphorylation : belongs to the kinase domain and can be phosphorylated by TBK1 itself, or by another serine kinase. This phosphorylation modifies the conformation of the (residues L164-G199), making the binding of the substrate possible. When S172 is not phosphorylated, the can dock itself between two αhelix of the kinase domain of another promoter. Furthermore, an containing the residues between D167 and L173 occupies the active site of this other protomer. Therefore, the active domain is not available for the binding of the substrate. But when S172 is phosphorylated, it can bind itself on the kinase domain of its own protomer, as the HPD domain which docks in the kinase domain in an intramolecular way, coming closer to the domain. This liberates the active site of the other protomer, which can now bind a substrate. ^[1]

The autophosphorylation between two subunit of a dimer is not really probable, since when the protein is dimeric, the two kinase domains are located at the opposite of one another. Therefore, the phosphorylation of Ser172 is done either by the concerned protomer, either by the kinase domain of another TBK1 dimer when TBK1 are involved in scaffolding complexes. ^[1] S172 can also be phosphorylated by kinases such as IKBKB or IKKB, and dephosphorylated by phosphatases such as PPM1B.

Polyubiquitination : Polyubiquitination in a Lys63 manner on

helps the activation of the kinase. The same type of modification on is responsible for dimerization. Type Lys48 polyubiquitination on Lys670 is done by DTX4 and is responsible for the degradation of the protein.

1 Signalling pathways
- 1.1 Inflammatory response
- 1.2 Anti-apoptosis
2 Diseases
3 References

Signalling pathways

Inflammatory response

The inflammatory response begin with the formation of the complex TBK1-TANK-TRAF. That allows phosphorylation of IRF3, IRF7 and DDX3X. Then these phosphorylated proteins form homodimers and they are translocated into the nucleus, where they activate transcription of interferon regulatory factors (IFN).

Anti-apoptosis

And the complex phosphorylates an inhibitor of NFkappaB, which finally activates NFkappaB, an anti-apoptotic transcription factor.

Diseases

Interaction with viral proteins :

TBK1 is the target of some viral proteins such as VP35 from Ebola virus ^[2] , or the P protein from Borna virus. Those proteins inhibit TBK1. Normally, TBK1 is responsible for virus-induced phosphorylations that lead to the cellular antiviral state. Therefore, the inhibition of the kinase prevents the establishment of such a cellular state. Since TBK1 is involved into the virus entrance following reaction, targeting TBK1 is a great solution for the viruses to prevent the reactions from the cell.

Oncology :

In case of some cancers, the permanent activation of TBK1 may be responsible of the proliferation of the cancerous cells. Since the substrates of TBK1 are involved in the proliferation pathway when phosphorylated, this permanent activation is a reason of the non apoptosis state of cells, and therefore of their proliferation.

References

↑ ^1.0 ^1.1 Ma X, Helgason E, Phung QT, Quan CL, Iyer RS, Lee MW, Bowman KK, Starovasnik MA, Dueber EC. Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9378-83. Epub 2012 May 22. PMID:22619329 doi:10.1073/pnas.1121552109
↑ Prins KC, Cardenas WB, Basler CF. Ebola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKKepsilon and TBK-1. J Virol. 2009 Apr;83(7):3069-77. doi: 10.1128/JVI.01875-08. Epub 2009 Jan 19. PMID:19153231 doi:http://dx.doi.org/10.1128/JVI.01875-08

Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox_Reserved_827"

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-= Overall structure =
+==Your Heading Here (maybe something like 'Structure')==<StructureSection load='1dq8' size='500' side='right' caption='Structure of HMG-CoA reductase (PDB entry [[1dq8]])' scene=''>= Overall structure =
 <Structure load='4IW0' size='500' frame='true' align='right' caption='TBK1 monomer' scene='56/568025/Vide/1' />
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 '''Polyubiquitination :'''  Polyubiquitination in a Lys63 manner on
-<scene name='56/568025/K30/1'>Lys30</scene> helps the activation of the kinase. The same type of modification on <scene name='56/568025/K401/1'>Lys401</scene> is responsible for dimerization. Type Lys48 polyubiquitination on Lys670 is done by DTX4 and is responsible for the degradation of the protein.
+<scene name='56/568025/K30/1'>Lys30</scene> helps the activation of the kinase. The same type of modification on <scene name='56/568025/K401/1'>Lys401</scene> is responsible for dimerization. Type Lys48 polyubiquitination on Lys670 is done by DTX4 and is responsible for the degradation of the protein.</StructureSection>
 = Signalling pathways =

Sandbox Reserved 827

From Proteopedia

Revision as of 15:23, 3 January 2014

Protomer

Dimer

Possible residue modifications

Contents

Signalling pathways

Inflammatory response

Anti-apoptosis

Diseases

References

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