2vaz
From Proteopedia
(New page: 200px<br /><applet load="2vaz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2vaz, resolution 10.0Å" /> '''MODEL OF THE S15-MRN...) |
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==Overview== | ==Overview== | ||
| - | Gene expression can be regulated at the level of initiation of protein | + | Gene expression can be regulated at the level of initiation of protein biosynthesis via structural elements present at the 5' untranslated region of mRNAs. These folded mRNA segments may bind to the ribosome, thus blocking translation until the mRNA unfolds. Here, we report a series of cryo-electron microscopy snapshots of ribosomal complexes directly visualizing either the mRNA structure blocked by repressor protein S15 or the unfolded, active mRNA. In the stalled state, the folded mRNA prevents the start codon from reaching the peptidyl-tRNA (P) site inside the ribosome. Upon repressor release, the mRNA unfolds and moves into the mRNA channel allowing translation initiation. A comparative structure and sequence analysis suggests the existence of a universal stand-by site on the ribosome (the 30S platform) dedicated for binding regulatory 5' mRNA elements. Different types of mRNA structures may be accommodated during translation preinitiation and regulate gene expression by transiently stalling the ribosome. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structured mRNAs | + | Structured mRNAs regulate translation initiation by binding to the platform of the ribosome., Marzi S, Myasnikov AG, Serganov A, Ehresmann C, Romby P, Yusupov M, Klaholz BP, Cell. 2007 Sep 21;130(6):1019-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889647 17889647] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ehresmann, C.]] | [[Category: Ehresmann, C.]] | ||
| - | [[Category: Klaholz, B | + | [[Category: Klaholz, B P.]] |
[[Category: Marzi, S.]] | [[Category: Marzi, S.]] | ||
| - | [[Category: Myasnikov, A | + | [[Category: Myasnikov, A G.]] |
[[Category: Romby, P.]] | [[Category: Romby, P.]] | ||
[[Category: Serganov, A.]] | [[Category: Serganov, A.]] | ||
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[[Category: translation initiation]] | [[Category: translation initiation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:54:41 2008'' |
Revision as of 16:54, 21 February 2008
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MODEL OF THE S15-MRNA COMPLEX FITTED INTO THE CRYO-EM MAP OF THE 70S ENTRAPMENT COMPLEX.
Overview
Gene expression can be regulated at the level of initiation of protein biosynthesis via structural elements present at the 5' untranslated region of mRNAs. These folded mRNA segments may bind to the ribosome, thus blocking translation until the mRNA unfolds. Here, we report a series of cryo-electron microscopy snapshots of ribosomal complexes directly visualizing either the mRNA structure blocked by repressor protein S15 or the unfolded, active mRNA. In the stalled state, the folded mRNA prevents the start codon from reaching the peptidyl-tRNA (P) site inside the ribosome. Upon repressor release, the mRNA unfolds and moves into the mRNA channel allowing translation initiation. A comparative structure and sequence analysis suggests the existence of a universal stand-by site on the ribosome (the 30S platform) dedicated for binding regulatory 5' mRNA elements. Different types of mRNA structures may be accommodated during translation preinitiation and regulate gene expression by transiently stalling the ribosome.
About this Structure
2VAZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structured mRNAs regulate translation initiation by binding to the platform of the ribosome., Marzi S, Myasnikov AG, Serganov A, Ehresmann C, Romby P, Yusupov M, Klaholz BP, Cell. 2007 Sep 21;130(6):1019-31. PMID:17889647
Page seeded by OCA on Thu Feb 21 18:54:41 2008
Categories: Escherichia coli | Protein complex | Ehresmann, C. | Klaholz, B P. | Marzi, S. | Myasnikov, A G. | Romby, P. | Serganov, A. | Yusupov, M. | Gene expression regulation | Mrna structure | Platform-binding center | Protein synthesis | Repressor protein | Ribonucleoprotein | Ribosomal protein | Ribosome | Riboswitch | Rna pseudoknot | Rna-binding | Rrna-binding | Translation initiation
