2veq
From Proteopedia
(New page: 200px<br /><applet load="2veq" size="350" color="white" frame="true" align="right" spinBox="true" caption="2veq, resolution 2.49Å" /> '''INSIGHTS INTO KINETO...) |
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==Overview== | ==Overview== | ||
| - | The CBF3 complex is an essential core component of the budding yeast | + | The CBF3 complex is an essential core component of the budding yeast kinetochore and is required for the centromeric localization of all other kinetochore proteins. We determined the crystal structure of a large section of the protein Cep3 from CBF3, which is the only component with obvious DNA-binding motifs. The protein adopts a roughly bilobal shape, with an extended dimerization interface. The dimer has a large central channel that is sufficient to accommodate duplex B-form DNA. The zinc-finger domains emerge at the edges of the channel, and could bind to the DNA in a pseudo-symmetrical manner at degenerate half-sites in the centromeric sequence. We propose a mechanism for the modulation of DNA affinity by an acidic activator domain, which could be applicable to a wider family of transcription factors. |
==About this Structure== | ==About this Structure== | ||
| - | 2VEQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=CAC:'>CAC</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC2:Cac Binding Site For Chain A'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEQ OCA]. | + | 2VEQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=CAC:'>CAC</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC2:Cac+Binding+Site+For+Chain+A'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEQ OCA]. |
==Reference== | ==Reference== | ||
| - | Insights into kinetochore-DNA interactions from the structure of Cep3Delta., Purvis A, Singleton MR, EMBO Rep. 2007 Dec 7 | + | Insights into kinetochore-DNA interactions from the structure of Cep3Delta., Purvis A, Singleton MR, EMBO Rep. 2008 Jan;9(1):56-62. Epub 2007 Dec 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18064045 18064045] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Purvis, A.]] | [[Category: Purvis, A.]] | ||
| - | [[Category: Singleton, M | + | [[Category: Singleton, M R.]] |
[[Category: BME]] | [[Category: BME]] | ||
[[Category: CAC]] | [[Category: CAC]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:55:23 2008'' |
Revision as of 16:55, 21 February 2008
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INSIGHTS INTO KINETOCHORE-DNA INTERACTIONS FROM THE STRUCTURE OF CEP3P
Overview
The CBF3 complex is an essential core component of the budding yeast kinetochore and is required for the centromeric localization of all other kinetochore proteins. We determined the crystal structure of a large section of the protein Cep3 from CBF3, which is the only component with obvious DNA-binding motifs. The protein adopts a roughly bilobal shape, with an extended dimerization interface. The dimer has a large central channel that is sufficient to accommodate duplex B-form DNA. The zinc-finger domains emerge at the edges of the channel, and could bind to the DNA in a pseudo-symmetrical manner at degenerate half-sites in the centromeric sequence. We propose a mechanism for the modulation of DNA affinity by an acidic activator domain, which could be applicable to a wider family of transcription factors.
About this Structure
2VEQ is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Insights into kinetochore-DNA interactions from the structure of Cep3Delta., Purvis A, Singleton MR, EMBO Rep. 2008 Jan;9(1):56-62. Epub 2007 Dec 7. PMID:18064045
Page seeded by OCA on Thu Feb 21 18:55:23 2008
