2yyj
From Proteopedia
(New page: 200px<br /><applet load="2yyj" size="350" color="white" frame="true" align="right" spinBox="true" caption="2yyj, resolution 1.66Å" /> '''Crystal structure of...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the | + | The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | Crystal | + | Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17804419 17804419] |
[[Category: 4-hydroxyphenylacetate 3-monooxygenase]] | [[Category: 4-hydroxyphenylacetate 3-monooxygenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 17: | Line 17: | ||
[[Category: Hisano, T.]] | [[Category: Hisano, T.]] | ||
[[Category: Iwasaki, W.]] | [[Category: Iwasaki, W.]] | ||
| - | [[Category: Kim, S | + | [[Category: Kim, S H.]] |
[[Category: Miki, K.]] | [[Category: Miki, K.]] | ||
[[Category: Takeda, K.]] | [[Category: Takeda, K.]] | ||
| Line 23: | Line 23: | ||
[[Category: FAD]] | [[Category: FAD]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| - | [[Category: 4-hydroxyphenylacetate 3-monooxygenase]] | ||
[[Category: fad and a substrate complex]] | [[Category: fad and a substrate complex]] | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| Line 31: | Line 30: | ||
[[Category: two-component flavin diffusible monooxygenase]] | [[Category: two-component flavin diffusible monooxygenase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:58:57 2008'' |
Revision as of 16:59, 21 February 2008
|
Crystal structure of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase complexed with FAD and 4-hydroxyphenylacetate
Overview
The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.
About this Structure
2YYJ is a Single protein structure of sequence from Thermus thermophilus with , and as ligands. Active as 4-hydroxyphenylacetate 3-monooxygenase, with EC number 1.14.13.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:17804419
Page seeded by OCA on Thu Feb 21 18:58:57 2008
Categories: 4-hydroxyphenylacetate 3-monooxygenase | Single protein | Thermus thermophilus | Ebihara, A. | Hisano, T. | Iwasaki, W. | Kim, S H. | Miki, K. | Takeda, K. | 4HP | FAD | SO4 | Fad and a substrate complex | Oxidoreductase | Oxygnase component | Riken spring-8 center | Structurome | Two-component flavin diffusible monooxygenase
