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2z35

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(New page: 200px<br /><applet load="2z35" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z35, resolution 2.2&Aring;" /> '''Crystal structure of ...)
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==Overview==
==Overview==
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All complexes of T cell receptors (TCRs) bound to peptide-major, histocompatibility complex (pMHC) molecules assume a stereotyped binding, 'polarity', despite wide variations in TCR-pMHC docking angles. However, existing TCR-pMHC crystal structures have failed to show broadly conserved, pairwise interaction motifs. Here we determined the crystal structures of, two TCRs encoded by the variable beta-chain 8.2 (V(beta)8.2), each bound, to the MHC class II molecule I-A(u), and did energetic mapping of V(alpha), and V(beta) contacts with I-A(u). Together with two previously solved, structures of V(beta)8.2-containing TCR-MHC complexes, we found four, TCR-I-A complexes with structurally superimposable interactions between, the V(beta) loops and the I-A alpha-helix. This examination of a narrow, 'slice' of the TCR-MHC repertoire demonstrates what is probably one of, many germline-derived TCR-MHC interaction 'codons'.
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All complexes of T cell receptors (TCRs) bound to peptide-major histocompatibility complex (pMHC) molecules assume a stereotyped binding 'polarity', despite wide variations in TCR-pMHC docking angles. However, existing TCR-pMHC crystal structures have failed to show broadly conserved pairwise interaction motifs. Here we determined the crystal structures of two TCRs encoded by the variable beta-chain 8.2 (V(beta)8.2), each bound to the MHC class II molecule I-A(u), and did energetic mapping of V(alpha) and V(beta) contacts with I-A(u). Together with two previously solved structures of V(beta)8.2-containing TCR-MHC complexes, we found four TCR-I-A complexes with structurally superimposable interactions between the V(beta) loops and the I-A alpha-helix. This examination of a narrow 'slice' of the TCR-MHC repertoire demonstrates what is probably one of many germline-derived TCR-MHC interaction 'codons'.
==About this Structure==
==About this Structure==
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Bond, C.J.]]
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[[Category: Bond, C J.]]
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[[Category: Ely, L.K.]]
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[[Category: Ely, L K.]]
[[Category: Feng, D.]]
[[Category: Feng, D.]]
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[[Category: Garcia, K.C.]]
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[[Category: Garcia, K C.]]
[[Category: immune receptor]]
[[Category: immune receptor]]
[[Category: immune system]]
[[Category: immune system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:06:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:59:46 2008''

Revision as of 16:59, 21 February 2008


2z35, resolution 2.2Å

Drag the structure with the mouse to rotate

Crystal structure of immune receptor

Overview

All complexes of T cell receptors (TCRs) bound to peptide-major histocompatibility complex (pMHC) molecules assume a stereotyped binding 'polarity', despite wide variations in TCR-pMHC docking angles. However, existing TCR-pMHC crystal structures have failed to show broadly conserved pairwise interaction motifs. Here we determined the crystal structures of two TCRs encoded by the variable beta-chain 8.2 (V(beta)8.2), each bound to the MHC class II molecule I-A(u), and did energetic mapping of V(alpha) and V(beta) contacts with I-A(u). Together with two previously solved structures of V(beta)8.2-containing TCR-MHC complexes, we found four TCR-I-A complexes with structurally superimposable interactions between the V(beta) loops and the I-A alpha-helix. This examination of a narrow 'slice' of the TCR-MHC repertoire demonstrates what is probably one of many germline-derived TCR-MHC interaction 'codons'.

About this Structure

2Z35 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural evidence for a germline-encoded T cell receptor-major histocompatibility complex interaction 'codon'., Feng D, Bond CJ, Ely LK, Maynard J, Garcia KC, Nat Immunol. 2007 Sep;8(9):975-83. Epub 2007 Aug 12. PMID:17694060

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