3k8z
From Proteopedia
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{{STRUCTURE_3k8z| PDB=3k8z | SCENE= }} | {{STRUCTURE_3k8z| PDB=3k8z | SCENE= }} | ||
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===Crystal Structure of Gudb1 a decryptified secondary glutamate dehydrogenase from B. subtilis=== | ===Crystal Structure of Gudb1 a decryptified secondary glutamate dehydrogenase from B. subtilis=== | ||
| + | {{ABSTRACT_PUBMED_20630473}} | ||
| - | + | ==Function== | |
| + | [[http://www.uniprot.org/uniprot/GUDB_BACSU GUDB_BACSU]] GudB seems to be intrinsically inactive, however spontaneous mutations removing a 9-bp direct repeat within the wild-type gudB sequence activated the GudB protein and allowed more-efficient utilization of amino acids of the glutamate family. This insertion presumably causes severe destabilization of the fold of the protein, leading to an inactive enzyme that is very quickly degraded. The cryptic GudB serves as a buffer that may compensate for mutations in the rocG gene and that can also be decryptified for the utilization of glutamate as a single carbon source in the absence of arginine. It is unable to synthesize glutamate.<ref>PMID:9829940</ref> <ref>PMID:18326565</ref> | ||
==About this Structure== | ==About this Structure== | ||
| - | [[3k8z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | [[3k8z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8Z OCA]. |
==See Also== | ==See Also== | ||
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==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:020630473</ref><references group="xtra"/> | + | <ref group="xtra">PMID:020630473</ref><references group="xtra"/><references/> |
| - | [[Category: Bacillus | + | [[Category: Bacillus globigii migula 1900]] |
[[Category: Glutamate dehydrogenase]] | [[Category: Glutamate dehydrogenase]] | ||
[[Category: Commichau, F M.]] | [[Category: Commichau, F M.]] | ||
Revision as of 06:20, 22 January 2014
Contents |
Crystal Structure of Gudb1 a decryptified secondary glutamate dehydrogenase from B. subtilis
Template:ABSTRACT PUBMED 20630473
Function
[GUDB_BACSU] GudB seems to be intrinsically inactive, however spontaneous mutations removing a 9-bp direct repeat within the wild-type gudB sequence activated the GudB protein and allowed more-efficient utilization of amino acids of the glutamate family. This insertion presumably causes severe destabilization of the fold of the protein, leading to an inactive enzyme that is very quickly degraded. The cryptic GudB serves as a buffer that may compensate for mutations in the rocG gene and that can also be decryptified for the utilization of glutamate as a single carbon source in the absence of arginine. It is unable to synthesize glutamate.[1] [2]
About this Structure
3k8z is a 6 chain structure with sequence from "bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900. Full crystallographic information is available from OCA.
See Also
Reference
- Gunka K, Newman JA, Commichau FM, Herzberg C, Rodrigues C, Hewitt L, Lewis RJ, Stulke J. Functional dissection of a trigger enzyme: mutations of the bacillus subtilis glutamate dehydrogenase RocG that affect differentially its catalytic activity and regulatory properties. J Mol Biol. 2010 Jul 23;400(4):815-27. Epub 2010 May 31. PMID:20630473 doi:10.1016/j.jmb.2010.05.055
- ↑ Belitsky BR, Sonenshein AL. Role and regulation of Bacillus subtilis glutamate dehydrogenase genes. J Bacteriol. 1998 Dec;180(23):6298-305. PMID:9829940
- ↑ Commichau FM, Gunka K, Landmann JJ, Stulke J. Glutamate metabolism in Bacillus subtilis: gene expression and enzyme activities evolved to avoid futile cycles and to allow rapid responses to perturbations of the system. J Bacteriol. 2008 May;190(10):3557-64. doi: 10.1128/JB.00099-08. Epub 2008 Mar 7. PMID:18326565 doi:http://dx.doi.org/10.1128/JB.00099-08
