2z9q
From Proteopedia
(New page: 200px<br /><applet load="2z9q" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z9q" /> '''Transfer RNA in the hybrid P/E state'''<br /...) |
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==Overview== | ==Overview== | ||
| - | During the ribosomal translocation, the binding of elongation factor G | + | During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions. |
==About this Structure== | ==About this Structure== | ||
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[[Category: twisted cca arm]] | [[Category: twisted cca arm]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:58 2008'' |
Revision as of 17:01, 21 February 2008
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Transfer RNA in the hybrid P/E state
Overview
During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
About this Structure
2Z9Q is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Locking and unlocking of ribosomal motions., Valle M, Zavialov A, Sengupta J, Rawat U, Ehrenberg M, Frank J, Cell. 2003 Jul 11;114(1):123-34. PMID:12859903
Page seeded by OCA on Thu Feb 21 19:00:58 2008
