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330d

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(New page: 200px<br /><applet load="330d" size="350" color="white" frame="true" align="right" spinBox="true" caption="330d, resolution 2.700&Aring;" /> '''BASE-PAIRING SHIFT ...)
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==Overview==
==Overview==
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the crystal packing of the B-DNA dodecamer, d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of, double helices with specific base-backbone interactions in the major, groove. Cooling the crystals below -10 degrees C stabilizes a new, conformational state with a long-range sequence-dependent one-step shift, in the major-groove base pairing. The tilt of the bases leads to the, disruption of the Watson-Crick pairing in the major groove and to the, formation of interactions with the 5' neighbour of their complement. This, alteration propagates along the helical axis over more than half a turn., As a result, the molecular structure is normal when seen from the minor, groove side and mismatched in the major groove. Comparison with a parent, isomorphous dodecamer structure corresponding to the codon 10-13 of the, c-Ha-ras proto-oncogene show that this new structural feature is sequence, dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are, involved both in recombination and in transcription, this new recognition, pattern should be considered in the analysis of the various processes, involving the reading of the genetic information.
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the crystal packing of the B-DNA dodecamer d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of double helices with specific base-backbone interactions in the major groove. Cooling the crystals below -10 degrees C stabilizes a new conformational state with a long-range sequence-dependent one-step shift in the major-groove base pairing. The tilt of the bases leads to the disruption of the Watson-Crick pairing in the major groove and to the formation of interactions with the 5' neighbour of their complement. This alteration propagates along the helical axis over more than half a turn. As a result, the molecular structure is normal when seen from the minor groove side and mismatched in the major groove. Comparison with a parent isomorphous dodecamer structure corresponding to the codon 10-13 of the c-Ha-ras proto-oncogene show that this new structural feature is sequence dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are involved both in recombination and in transcription, this new recognition pattern should be considered in the analysis of the various processes involving the reading of the genetic information.
==About this Structure==
==About this Structure==
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:35:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:05 2008''

Revision as of 17:02, 21 February 2008

Image:330d.gif

330d, resolution 2.700Å

Drag the structure with the mouse to rotate

BASE-PAIRING SHIFT IN THE MAJOR GROOVE OF (CA)N TRACTS BY B-DNA CRYSTAL STRUCTURES

Overview

the crystal packing of the B-DNA dodecamer d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of double helices with specific base-backbone interactions in the major groove. Cooling the crystals below -10 degrees C stabilizes a new conformational state with a long-range sequence-dependent one-step shift in the major-groove base pairing. The tilt of the bases leads to the disruption of the Watson-Crick pairing in the major groove and to the formation of interactions with the 5' neighbour of their complement. This alteration propagates along the helical axis over more than half a turn. As a result, the molecular structure is normal when seen from the minor groove side and mismatched in the major groove. Comparison with a parent isomorphous dodecamer structure corresponding to the codon 10-13 of the c-Ha-ras proto-oncogene show that this new structural feature is sequence dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are involved both in recombination and in transcription, this new recognition pattern should be considered in the analysis of the various processes involving the reading of the genetic information.

About this Structure

330D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Base-pairing shift in the major groove of (CA)n tracts by B-DNA crystal structures., Timsit Y, Vilbois E, Moras D, Nature. 1991 Nov 14;354(6349):167-70. PMID:1944598

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