353d
From Proteopedia
(New page: 200px<br /><applet load="353d" size="350" color="white" frame="true" align="right" spinBox="true" caption="353d, resolution 2.400Å" /> '''CRYSTAL STRUCTURE O...) |
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==Overview== | ==Overview== | ||
| - | This is the first high resolution crystal structure of an RNA molecule | + | This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 A including 159 solvent molecules. Most of the 2'-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stabilizing an RNA structure. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Bald, R.]] | [[Category: Bald, R.]] | ||
[[Category: Betzel, C.]] | [[Category: Betzel, C.]] | ||
| - | [[Category: Erdmann, V | + | [[Category: Erdmann, V A.]] |
| - | [[Category: Furste, J | + | [[Category: Furste, J P.]] |
[[Category: Lorenz, S.]] | [[Category: Lorenz, S.]] | ||
| - | [[Category: Schneider, T | + | [[Category: Schneider, T R.]] |
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
[[Category: a-rna]] | [[Category: a-rna]] | ||
[[Category: double strand]] | [[Category: double strand]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:14 2008'' |
Revision as of 17:02, 21 February 2008
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CRYSTAL STRUCTURE OF DOMAIN A OF THERMUS FLAVUS 5S RRNA AND THE CONTRIBUTION OF WATER MOLECULES TO ITS STRUCTURE
Overview
This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 A including 159 solvent molecules. Most of the 2'-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stabilizing an RNA structure.
About this Structure
353D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of domain A of Thermus flavus 5S rRNA and the contribution of water molecules to its structure., Betzel C, Lorenz S, Furste JP, Bald R, Zhang M, Schneider TR, Wilson KS, Erdmann VA, FEBS Lett. 1994 Sep 5;351(2):159-64. PMID:8082756
Page seeded by OCA on Thu Feb 21 19:02:14 2008
