357d
From Proteopedia
(New page: 200px<br /><applet load="357d" size="350" color="white" frame="true" align="right" spinBox="true" caption="357d, resolution 3.500Å" /> '''3.5 A STRUCTURE OF ...) |
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==Overview== | ==Overview== | ||
| - | Two new RNA structures portray how non-Watson-Crick base pairs and metal | + | Two new RNA structures portray how non-Watson-Crick base pairs and metal ions can produce a unique RNA shape suitable for recognition by proteins. The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a duplex dodecamer encompassing an internal loop E have been determined at 3.0 and 1.5 A, respectively. This loop E region is distorted by three "cross-strand purine stacks" and three novel, water-mediated noncanonical base pairs and stabilized by a four metal ion zipper. These features give its minor groove a unique hydrogen-bonding surface and make the adjacent major groove wide enough to permit recognition by the ribosomal protein L25, which is expected to bind to this surface. |
==About this Structure== | ==About this Structure== | ||
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[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Correll, C | + | [[Category: Correll, C C.]] |
[[Category: Freeborn, B.]] | [[Category: Freeborn, B.]] | ||
| - | [[Category: Moore, P | + | [[Category: Moore, P B.]] |
| - | [[Category: Steitz, T | + | [[Category: Steitz, T A.]] |
[[Category: HG]] | [[Category: HG]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: u-rna]] | [[Category: u-rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:16 2008'' |
Revision as of 17:02, 21 February 2008
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3.5 A STRUCTURE OF FRAGMENT I FROM E. COLI 5S RRNA
Overview
Two new RNA structures portray how non-Watson-Crick base pairs and metal ions can produce a unique RNA shape suitable for recognition by proteins. The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a duplex dodecamer encompassing an internal loop E have been determined at 3.0 and 1.5 A, respectively. This loop E region is distorted by three "cross-strand purine stacks" and three novel, water-mediated noncanonical base pairs and stabilized by a four metal ion zipper. These features give its minor groove a unique hydrogen-bonding surface and make the adjacent major groove wide enough to permit recognition by the ribosomal protein L25, which is expected to bind to this surface.
About this Structure
357D is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain., Correll CC, Freeborn B, Moore PB, Steitz TA, Cell. 1997 Nov 28;91(5):705-12. PMID:9393863 [[Category: ]]
Page seeded by OCA on Thu Feb 21 19:02:16 2008
Categories: Protein complex | Correll, C C. | Freeborn, B. | Moore, P B. | Steitz, T A. | HG | MG | Double helix | Internal loop | Kinked | Mismatched | Modified | Overhanging base | U-rna
