359d
From Proteopedia
(New page: 200px<br /><applet load="359d" size="350" color="white" frame="true" align="right" spinBox="true" caption="359d, resolution 2.900Å" /> '''INHIBITION OF THE H...) |
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==Overview== | ==Overview== | ||
| - | Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by | + | Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state. |
==About this Structure== | ==About this Structure== | ||
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Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9417029 9417029] | Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9417029 9417029] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Feig, A | + | [[Category: Feig, A L.]] |
| - | [[Category: Scott, W | + | [[Category: Scott, W G.]] |
| - | [[Category: Uhlenbeck, O | + | [[Category: Uhlenbeck, O C.]] |
[[Category: TB]] | [[Category: TB]] | ||
[[Category: catalytic rna]] | [[Category: catalytic rna]] | ||
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[[Category: rna hammerhead ribozyme]] | [[Category: rna hammerhead ribozyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:25 2008'' |
Revision as of 17:02, 21 February 2008
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INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)
Overview
Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.
About this Structure
359D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:9417029
Page seeded by OCA on Thu Feb 21 19:02:25 2008
