377d
From Proteopedia
(New page: 200px<br /><applet load="377d" size="350" color="white" frame="true" align="right" spinBox="true" caption="377d, resolution 1.760Å" /> '''5'-R(*CP*GP*UP*AP*C...) |
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==Overview== | ==Overview== | ||
| - | The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal | + | The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Biswas, R.]] | [[Category: Biswas, R.]] | ||
| - | [[Category: Mitra, S | + | [[Category: Mitra, S N.]] |
[[Category: Sundaralingam, M.]] | [[Category: Sundaralingam, M.]] | ||
[[Category: a-rna]] | [[Category: a-rna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:28 2008'' |
Revision as of 17:02, 21 February 2008
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5'-R(*CP*GP*UP*AP*CP*G)-3'
Overview
The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes.
About this Structure
377D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
1.76 A structure of a pyrimidine start alternating A-RNA hexamer r(CGUAC)dG., Biswas R, Mitra SN, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):570-6. PMID:9761851
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