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3aji
From Proteopedia
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| - | [[Image:3aji.png|left|200px]] | ||
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{{STRUCTURE_3aji| PDB=3aji | SCENE= }} | {{STRUCTURE_3aji| PDB=3aji | SCENE= }} | ||
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===Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion=== | ===Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion=== | ||
| + | {{ABSTRACT_PUBMED_21128684}} | ||
| - | + | ==Function== | |
| + | [[http://www.uniprot.org/uniprot/PSD10_MOUSE PSD10_MOUSE]] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. Acts as an oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export (By similarity). [[http://www.uniprot.org/uniprot/PRS6B_MOUSE PRS6B_MOUSE]] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. | ||
==About this Structure== | ==About this Structure== | ||
| - | [[3aji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | [[3aji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AJI OCA]. |
==See Also== | ==See Also== | ||
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==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:021128684</ref><references group="xtra"/> | + | <ref group="xtra">PMID:021128684</ref><references group="xtra"/><references/> |
| - | [[Category: | + | [[Category: Lk3 transgenic mice]] |
[[Category: Hino, N.]] | [[Category: Hino, N.]] | ||
[[Category: Mimasu, S.]] | [[Category: Mimasu, S.]] | ||
Revision as of 06:01, 29 January 2014
Contents |
Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion
Template:ABSTRACT PUBMED 21128684
Function
[PSD10_MOUSE] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. Acts as an oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export (By similarity). [PRS6B_MOUSE] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
About this Structure
3aji is a 4 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA.
See Also
Reference
- Sato S, Mimasu S, Sato A, Hino N, Sakamoto K, Umehara T, Yokoyama S. Crystallographic Study of a Site-Specifically Cross-Linked Protein Complex with a Genetically Incorporated Photoreactive Amino Acid . Biochemistry. 2010 Dec 16. PMID:21128684 doi:10.1021/bi1016183
Categories: Lk3 transgenic mice | Hino, N. | Mimasu, S. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sakamoto, K. | Sato, A. | Sato, S. | Umehara, T. | Yokoyama, S. | Amber suppression | Chaperone-protein binding complex | Gankyrin | P-benzoyl-l-phenylalanine | Pbpa | Riken structural genomics/proteomics initiative | Rsgi | S6 atpase | Structural genomic
