398d
From Proteopedia
(New page: 200px<br /> <applet load="398d" size="450" color="white" frame="true" align="right" spinBox="true" caption="398d, resolution 1.940Å" /> '''3'-DNA-RNA-5' JUNC...) |
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| - | [[Image:398d.gif|left|200px]]<br /> | + | [[Image:398d.gif|left|200px]]<br /><applet load="398d" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="398d" size=" | + | |
caption="398d, resolution 1.940Å" /> | caption="398d, resolution 1.940Å" /> | ||
'''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''<br /> | '''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During initiation of minus-strand synthesis by HIV-1 reverse | + | During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H. |
==About this Structure== | ==About this Structure== | ||
| - | 398D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 398D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:37 2008'' |
Revision as of 17:02, 21 February 2008
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3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION
Overview
During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.
About this Structure
398D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication., Mueller U, Maier G, Mochi Onori A, Cellai L, Heumann H, Heinemann U, Biochemistry. 1998 Sep 1;37(35):12005-11. PMID:9724510
Page seeded by OCA on Thu Feb 21 19:02:37 2008
