399d
From Proteopedia
(New page: 200px<br /><applet load="399d" size="350" color="white" frame="true" align="right" spinBox="true" caption="399d, resolution 1.9Å" /> '''STRUCTURE OF D(CGCCCG...) |
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==Overview== | ==Overview== | ||
| - | We have determined the structure of the dodecamer duplex d(CGCCCGCGGGCG)2. | + | We have determined the structure of the dodecamer duplex d(CGCCCGCGGGCG)2. A careful use of the molecular replacement programme AMoRe has been essential in order to solve the structure. This dodecamer shows a unique conformation, quite different from all the previously studied oligonucleotide duplexes: the central octamer has an A conformation, but with a sharp 65 degrees kink in the centre; the terminal base-steps have a B-like conformation; the major groove is completely closed in the centre, a hollow molecule is thus found. The results obtained confirm the high degree of variability of DNA structure. A new type of kink and an intermediate A/B double-helical conformation have been found. Such intermediate conformation differs from those described in DNA polymerase complexes. |
==About this Structure== | ==About this Structure== | ||
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Structure of the d(CGCCCGCGGGCG) dodecamer: a kinked A-DNA molecule showing some B-DNA features., Malinina L, Fernandez LG, Huynh-Dinh T, Subirana JA, J Mol Biol. 1999 Jan 29;285(4):1679-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9917405 9917405] | Structure of the d(CGCCCGCGGGCG) dodecamer: a kinked A-DNA molecule showing some B-DNA features., Malinina L, Fernandez LG, Huynh-Dinh T, Subirana JA, J Mol Biol. 1999 Jan 29;285(4):1679-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9917405 9917405] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Fernandez, L | + | [[Category: Fernandez, L G.]] |
| - | [[Category: Malinina, J | + | [[Category: Malinina, J A.Subirana L.]] |
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:38 2008'' |
Revision as of 17:02, 21 February 2008
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STRUCTURE OF D(CGCCCGCGGGCG)
Overview
We have determined the structure of the dodecamer duplex d(CGCCCGCGGGCG)2. A careful use of the molecular replacement programme AMoRe has been essential in order to solve the structure. This dodecamer shows a unique conformation, quite different from all the previously studied oligonucleotide duplexes: the central octamer has an A conformation, but with a sharp 65 degrees kink in the centre; the terminal base-steps have a B-like conformation; the major groove is completely closed in the centre, a hollow molecule is thus found. The results obtained confirm the high degree of variability of DNA structure. A new type of kink and an intermediate A/B double-helical conformation have been found. Such intermediate conformation differs from those described in DNA polymerase complexes.
About this Structure
399D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the d(CGCCCGCGGGCG) dodecamer: a kinked A-DNA molecule showing some B-DNA features., Malinina L, Fernandez LG, Huynh-Dinh T, Subirana JA, J Mol Biol. 1999 Jan 29;285(4):1679-90. PMID:9917405
Page seeded by OCA on Thu Feb 21 19:02:38 2008
