3b4c
From Proteopedia
(New page: 200px<br /><applet load="3b4c" size="350" color="white" frame="true" align="right" spinBox="true" caption="3b4c, resolution 3.0Å" /> '''T. tengcongensis glmS...) |
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==Overview== | ==Overview== | ||
| - | The glmS ribozyme is a catalytic riboswitch that is activated for | + | The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermoanaerobacter tengcongensis]] | [[Category: Thermoanaerobacter tengcongensis]] | ||
| - | [[Category: Amare, A | + | [[Category: Amare, A R.Ferre-D.]] |
| - | [[Category: Klein, D | + | [[Category: Klein, D J.]] |
[[Category: 3AD]] | [[Category: 3AD]] | ||
[[Category: GLP]] | [[Category: GLP]] | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:03:16 2008'' |
Revision as of 17:03, 21 February 2008
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T. tengcongensis glmS ribozyme bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage
Overview
The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
About this Structure
3B4C is a Protein complex structure of sequences from Thermoanaerobacter tengcongensis with , and as ligands. Full crystallographic information is available from OCA.
Reference
Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:17990888
Page seeded by OCA on Thu Feb 21 19:03:16 2008
