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2blz

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[[Category: synchrotron]]
[[Category: synchrotron]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:51:43 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:42:05 2007''

Revision as of 14:37, 30 October 2007

Image:2blz.gif

2blz, resolution 1.40Å

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RNASE AFTER A HIGH DOSE X-RAY "BURN"

Overview

Specific radiation damage can be used to solve macromolecular structures, using the radiation-damage-induced phasing (RIP) method. The method has, been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets, that were collected on a third-generation synchrotron undulator beamline, with a highly attenuated beam. Each crystal was exposed to the, unattenuated X-ray beam between the collection of a 'before' and an, 'after' data set. The X-ray 'burn'-induced intensity differences ranged, from 5 to 15%, depending on the protein investigated. X-ray-susceptible, substructures were determined using the integrated direct and Patterson, methods in SHELXD. The best substructures were found by downscaling the, ... [(full description)]

About this Structure

2BLZ is a [Single protein] structure of sequence from [Bos taurus] with CL as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Improving radiation-damage substructures for RIP., Nanao MH, Sheldrick GM, Ravelli RB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756

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OCA

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