2bm1
From Proteopedia
Revision as of 14:37, 30 October 2007
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RIBOSOMAL ELONGATION FACTOR G (EF-G) FUSIDIC ACID RESISTANT MUTANT G16V
Overview
Fusidic acid (FA) is a steroid antibiotic commonly used against Gram, positive bacterial infections. It inhibits protein synthesis by stalling, elongation factor G (EF-G) on the ribosome after translocation. A, significant number of the mutations conferring strong FA resistance have, been mapped at the interfaces between domains G, III and V of EF-G., However, direct information on how such mutations affect the structure has, hitherto not been available. Here we present the crystal structures of two, mutants of Thermus thermophilus EF-G, G16V and T84A, which exhibit FA, hypersensitivity and resistance in vitro, respectively. These mutants also, have higher and lower affinity for GTP respectively than wild-type EF-G., The mutations cause significant conformational changes in the switch II, ... [(full description)]
About this Structure
2BM1 is a [Single protein] structure of sequence from [Thermus thermophilus] with MG and GDP as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural insights into fusidic acid resistance and sensitivity in EF-G., Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT, J Mol Biol. 2005 May 13;348(4):939-49. PMID:15843024
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