2bm3

From Proteopedia

Revision as of 14:37, 30 October 2007

STRUCTURE OF THE TYPE II COHESIN FROM CLOSTRIDIUM THERMOCELLUM SDBA

Overview

The plant cell wall degrading enzymes expressed by anaerobic, microorganisms form large multienzyme complexes (cellulosomes)., Cellulosomes assemble by the Type I dockerins on the catalytic subunits, binding to the reiterated Type I cohesins in the molecular scaffold, while, Type II dockerin-cohesin interactions anchor the complex onto the, bacterial cell surface. Type I and Type II cohesin, dockerin pairs show no, cross-specificity. Here we report the crystal structure of the Type II, cohesin (CohII) from the Clostridium thermocellum cell surface anchoring, protein SdbA. The protein domain contains nine beta-strands and a small, alpha-helix. The beta-strands assemble into two elongated beta-sheets that, display a typical jelly roll fold. The structure of CohII is very similar, to Type I ... [(full description)]

About this Structure

2BM3 is a [Single protein] structure of sequence from [Clostridium thermocellum] with IPA as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Insights into the structural determinants of cohesin-dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA., Carvalho AL, Pires VM, Gloster TM, Turkenburg JP, Prates JA, Ferreira LM, Romao MJ, Davies GJ, Fontes CM, Gilbert HJ, J Mol Biol. 2005 Jun 24;349(5):909-15. PMID:15913653

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