3bci
From Proteopedia
(New page: 200px<br /><applet load="3bci" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bci, resolution 1.810Å" /> '''Crystal Structure o...) |
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==Overview== | ==Overview== | ||
| - | Bacterial Dsb proteins catalyse the in vivo formation of disulfide bonds, a critical step in the stability and activity of many proteins. Most | + | Bacterial Dsb proteins catalyse the in vivo formation of disulfide bonds, a critical step in the stability and activity of many proteins. Most studies on Dsb proteins have focused on Gram-negative bacteria and thus the process of oxidative folding in Gram-positive bacteria is poorly understood. To help elucidate this process in Gram-positive bacteria, DsbA from Staphylococcus aureus (SaDsbA) has been focused on. Here, the expression, purification, crystallization and preliminary diffraction analysis of SaDsbA are reported. SaDsbA crystals diffract to a resolution limit of 2.1 A and belong to the hexagonal space group P6(5) or P6(1), with unit-cell parameters a = b = 72.1, c = 92.1 A and one molecule in the asymmetric unit (64% solvent content). |
==About this Structure== | ==About this Structure== | ||
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[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Heras, B.]] | [[Category: Heras, B.]] | ||
| - | [[Category: Martin, J | + | [[Category: Martin, J L.]] |
[[Category: Thony-Meyer, L.]] | [[Category: Thony-Meyer, L.]] | ||
[[Category: protein folding]] | [[Category: protein folding]] | ||
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[[Category: thiol-disulfide oxidoreductase]] | [[Category: thiol-disulfide oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:04:59 2008'' |
Revision as of 17:05, 21 February 2008
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Crystal Structure of Staphylococcus aureus DsbA
Overview
Bacterial Dsb proteins catalyse the in vivo formation of disulfide bonds, a critical step in the stability and activity of many proteins. Most studies on Dsb proteins have focused on Gram-negative bacteria and thus the process of oxidative folding in Gram-positive bacteria is poorly understood. To help elucidate this process in Gram-positive bacteria, DsbA from Staphylococcus aureus (SaDsbA) has been focused on. Here, the expression, purification, crystallization and preliminary diffraction analysis of SaDsbA are reported. SaDsbA crystals diffract to a resolution limit of 2.1 A and belong to the hexagonal space group P6(5) or P6(1), with unit-cell parameters a = b = 72.1, c = 92.1 A and one molecule in the asymmetric unit (64% solvent content).
About this Structure
3BCI is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Expression and crystallization of DsbA from Staphylococcus aureus., Heras B, Kurz M, Jarrott R, Byriel KA, Jones A, Thony-Meyer L, Martin JL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt, 11):953-6. Epub 2007 Oct 24. PMID:18007049
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