3bes
From Proteopedia
(New page: 200px<br /><applet load="3bes" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bes, resolution 2.2Å" /> '''Structure of a Poxvir...) |
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==Overview== | ==Overview== | ||
- | Ectromelia virus (ECTV) encodes an IFN-gamma-binding protein | + | Ectromelia virus (ECTV) encodes an IFN-gamma-binding protein (IFN-gammaBP(ECTV)) that disrupts IFN-gamma signaling and its ability to induce an antiviral state within cells. IFN-gammaBP(ECTV) is an important virulence factor that is highly conserved (>90%) in all orthopoxviruses, including variola virus, the causative agent of smallpox. The 2.2-A crystal structure of the IFN-gammaBP(ECTV)/IFN-gamma complex reveals IFN-gammaBP(ECTV) consists of an IFN-gammaR1 ligand-binding domain and a 57-aa helix-turn-helix (HTH) motif that is structurally related to the transcription factor TFIIA. The HTH motif forms a tetramerization domain that results in an IFN-gammaBP(ECTV)/IFN-gamma complex containing four IFN-gammaBP(ECTV) chains and two IFN-gamma dimers. The structure, combined with biochemical and cell-based assays, demonstrates that IFN-gammaBP(ECTV) tetramers are required for efficient IFN-gamma antagonism. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
- | Structure and mechanism of IFN- | + | Structure and mechanism of IFN-gamma antagonism by an orthopoxvirus IFN-gamma-binding protein., Nuara AA, Walter LJ, Logsdon NJ, Yoon SI, Jones BC, Schriewer JM, Buller RM, Walter MR, Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1861-6. Epub 2008 Feb 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18252829 18252829] |
[[Category: Ectromelia virus]] | [[Category: Ectromelia virus]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
- | [[Category: Nuara, A | + | [[Category: Nuara, A A]] |
- | [[Category: Walter, M | + | [[Category: Walter, M R.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
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[[Category: receptor]] | [[Category: receptor]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:05:21 2008'' |
Revision as of 17:05, 21 February 2008
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Structure of a Poxvirus ifngbp/ifng Complex
Overview
Ectromelia virus (ECTV) encodes an IFN-gamma-binding protein (IFN-gammaBP(ECTV)) that disrupts IFN-gamma signaling and its ability to induce an antiviral state within cells. IFN-gammaBP(ECTV) is an important virulence factor that is highly conserved (>90%) in all orthopoxviruses, including variola virus, the causative agent of smallpox. The 2.2-A crystal structure of the IFN-gammaBP(ECTV)/IFN-gamma complex reveals IFN-gammaBP(ECTV) consists of an IFN-gammaR1 ligand-binding domain and a 57-aa helix-turn-helix (HTH) motif that is structurally related to the transcription factor TFIIA. The HTH motif forms a tetramerization domain that results in an IFN-gammaBP(ECTV)/IFN-gamma complex containing four IFN-gammaBP(ECTV) chains and two IFN-gamma dimers. The structure, combined with biochemical and cell-based assays, demonstrates that IFN-gammaBP(ECTV) tetramers are required for efficient IFN-gamma antagonism.
About this Structure
3BES is a Protein complex structure of sequences from Ectromelia virus and Homo sapiens with and as ligands. Known structural/functional Sites: , , , , , and . Full crystallographic information is available from OCA.
Reference
Structure and mechanism of IFN-gamma antagonism by an orthopoxvirus IFN-gamma-binding protein., Nuara AA, Walter LJ, Logsdon NJ, Yoon SI, Jones BC, Schriewer JM, Buller RM, Walter MR, Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1861-6. Epub 2008 Feb 5. PMID:18252829
Page seeded by OCA on Thu Feb 21 19:05:21 2008
Categories: Ectromelia virus | Homo sapiens | Protein complex | Nuara, A A | Walter, M R. | NAG | PO4 | Antiviral defense | Cytokine | Glycoprotein | Immune system | Interferon | Orthopoxvirus | Receptor