3bes

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(New page: 200px<br /><applet load="3bes" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bes, resolution 2.2&Aring;" /> '''Structure of a Poxvir...)
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==Overview==
==Overview==
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Ectromelia virus (ECTV) encodes an IFN-gamma-binding protein, (IFN-gammaBP(ECTV)) that disrupts IFN-gamma signaling and its ability to, induce an antiviral state within cells. IFN-gammaBP(ECTV) is an important, virulence factor that is highly conserved (&gt;90%) in all orthopoxviruses, including variola virus, the causative agent of smallpox. The 2.2-A, crystal structure of the IFN-gammaBP(ECTV)/IFN-gamma complex reveals, IFN-gammaBP(ECTV) consists of an IFN-gammaR1 ligand-binding domain and a, 57-aa helix-turn-helix (HTH) motif that is structurally related to the, transcription factor TFIIA. The HTH motif forms a tetramerization domain, that results in an IFN-gammaBP(ECTV)/IFN-gamma complex containing four, IFN-gammaBP(ECTV) chains and two IFN-gamma dimers. The structure, combined, with biochemical and cell-based assays, demonstrates that, IFN-gammaBP(ECTV) tetramers are required for efficient IFN-gamma, antagonism.
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Ectromelia virus (ECTV) encodes an IFN-gamma-binding protein (IFN-gammaBP(ECTV)) that disrupts IFN-gamma signaling and its ability to induce an antiviral state within cells. IFN-gammaBP(ECTV) is an important virulence factor that is highly conserved (&gt;90%) in all orthopoxviruses, including variola virus, the causative agent of smallpox. The 2.2-A crystal structure of the IFN-gammaBP(ECTV)/IFN-gamma complex reveals IFN-gammaBP(ECTV) consists of an IFN-gammaR1 ligand-binding domain and a 57-aa helix-turn-helix (HTH) motif that is structurally related to the transcription factor TFIIA. The HTH motif forms a tetramerization domain that results in an IFN-gammaBP(ECTV)/IFN-gamma complex containing four IFN-gammaBP(ECTV) chains and two IFN-gamma dimers. The structure, combined with biochemical and cell-based assays, demonstrates that IFN-gammaBP(ECTV) tetramers are required for efficient IFN-gamma antagonism.
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Structure and mechanism of IFN-{gamma} antagonism by an orthopoxvirus IFN-{gamma}-binding protein., Nuara AA, Walter LJ, Logsdon NJ, Yoon SI, Jones BC, Schriewer JM, Buller RM, Walter MR, Proc Natl Acad Sci U S A. 2008 Feb 5;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18252829 18252829]
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Structure and mechanism of IFN-gamma antagonism by an orthopoxvirus IFN-gamma-binding protein., Nuara AA, Walter LJ, Logsdon NJ, Yoon SI, Jones BC, Schriewer JM, Buller RM, Walter MR, Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1861-6. Epub 2008 Feb 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18252829 18252829]
[[Category: Ectromelia virus]]
[[Category: Ectromelia virus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Nuara, A.A]]
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[[Category: Nuara, A A]]
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[[Category: Walter, M.R.]]
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[[Category: Walter, M R.]]
[[Category: NAG]]
[[Category: NAG]]
[[Category: PO4]]
[[Category: PO4]]
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[[Category: receptor]]
[[Category: receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:23:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:05:21 2008''

Revision as of 17:05, 21 February 2008


3bes, resolution 2.2Å

Drag the structure with the mouse to rotate

Structure of a Poxvirus ifngbp/ifng Complex

Overview

Ectromelia virus (ECTV) encodes an IFN-gamma-binding protein (IFN-gammaBP(ECTV)) that disrupts IFN-gamma signaling and its ability to induce an antiviral state within cells. IFN-gammaBP(ECTV) is an important virulence factor that is highly conserved (>90%) in all orthopoxviruses, including variola virus, the causative agent of smallpox. The 2.2-A crystal structure of the IFN-gammaBP(ECTV)/IFN-gamma complex reveals IFN-gammaBP(ECTV) consists of an IFN-gammaR1 ligand-binding domain and a 57-aa helix-turn-helix (HTH) motif that is structurally related to the transcription factor TFIIA. The HTH motif forms a tetramerization domain that results in an IFN-gammaBP(ECTV)/IFN-gamma complex containing four IFN-gammaBP(ECTV) chains and two IFN-gamma dimers. The structure, combined with biochemical and cell-based assays, demonstrates that IFN-gammaBP(ECTV) tetramers are required for efficient IFN-gamma antagonism.

About this Structure

3BES is a Protein complex structure of sequences from Ectromelia virus and Homo sapiens with and as ligands. Known structural/functional Sites: , , , , , and . Full crystallographic information is available from OCA.

Reference

Structure and mechanism of IFN-gamma antagonism by an orthopoxvirus IFN-gamma-binding protein., Nuara AA, Walter LJ, Logsdon NJ, Yoon SI, Jones BC, Schriewer JM, Buller RM, Walter MR, Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1861-6. Epub 2008 Feb 5. PMID:18252829

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