4bth

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{{STRUCTURE_4bth| PDB=4bth | SCENE= }}
{{STRUCTURE_4bth| PDB=4bth | SCENE= }}
===The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains===
===The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains===
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{{ABSTRACT_PUBMED_24474783}}
==Function==
==Function==
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==Reference==
==Reference==
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<references group="xtra"/><references/>
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<ref group="xtra">PMID:024474783</ref><references group="xtra"/><references/>
[[Category: Acyl-homoserine-lactone acylase]]
[[Category: Acyl-homoserine-lactone acylase]]
[[Category: Bokhove, M.]]
[[Category: Bokhove, M.]]

Revision as of 03:22, 13 February 2014

Template:STRUCTURE 4bth

Contents

The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains

Template:ABSTRACT PUBMED 24474783

Function

[PVDQ_PSEAE] Catalyzes the deacylation of acyl-homoserine lactone (AHL or acyl-HSL), releasing homoserine lactone (HSL) and the corresponding fatty acid. Possesses a specificity for the degradation of long-chain acyl-HSLs (side chains of 11 to 14 carbons in length). Degrades 3-oxo-C12-HSL, one of the two main AHL signal molecules of P.aeruginosa, and thereby functions as a quorum quencher, inhibiting the las quorum-sensing system. Therefore, may enable P.aeruginosa to modulate its own quorum-sensing-dependent pathogenic potential. Also appears to be required for pyoverdin biosynthesis.[1] [2] [3]

About this Structure

4bth is a 2 chain structure. Full crystallographic information is available from OCA.

Reference

  • Koch G, Nadal-Jimenez P, Reis CR, Muntendam R, Bokhove M, Melillo E, Dijkstra BW, Cool RH, Quax WJ. Reducing virulence of the human pathogen Burkholderia by altering the substrate specificity of the quorum-quenching acylase PvdQ. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1568-73. doi:, 10.1073/pnas.1311263111. Epub 2014 Jan 13. PMID:24474783 doi:http://dx.doi.org/10.1073/pnas.1311263111
  1. ↑ Sio CF, Otten LG, Cool RH, Diggle SP, Braun PG, Bos R, Daykin M, Camara M, Williams P, Quax WJ. Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1. Infect Immun. 2006 Mar;74(3):1673-82. PMID:16495538 doi:74/3/1673
  2. ↑ Huang JJ, Han JI, Zhang LH, Leadbetter JR. Utilization of acyl-homoserine lactone quorum signals for growth by a soil pseudomonad and Pseudomonas aeruginosa PAO1. Appl Environ Microbiol. 2003 Oct;69(10):5941-9. PMID:14532048
  3. ↑ Lamont IL, Martin LW. Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa. Microbiology. 2003 Apr;149(Pt 4):833-42. PMID:12686626

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