Catabolite gene activator protein

From Proteopedia

Revision as of 12:47, 17 February 2014

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Catabolite gene activator protein (CAP) is a DNA-binding protein involved in the transcription of several genes including those involved in the metabolism of sugar. CAP can activate or repress transcription initiation upon binding of cAMP. The cAMP-CAP complex binds to promoters on the DNA. The structure contains a ligand-binding domain (LBD) at the N-terminal and DNA-binding domain (DBD) at the C-terminal. Two cAMP molecules bind to the dimeric CAP. When the amount of glucose transported into the cell is low, cAMP level rises and the cAMP-CAP complex causes increase of transcription of glucose metabolism genes.

3D Structures of catabolite gene activator protein

Updated on 17-February-2014

3d0s – MtCAP – Mycobacterium tuberculosis
2wc2 – EcCAP – Escherichia coli - NMR
3hif, 4ft8, 4hzf – EcCAP
3fwe, 4bh9, 4bhp – EcCAP (mutant)

CAP complex with cAMP

1g6n, 1i5z, 2gzw, 3ryp, 3ryr, 4i01, 4i02, 4i09, 4i0a, 4i0b – EcCAP + cAMP
1hw5, 1i6x, 3kcc, 3qop, 3rdi, 3rou, 3rpq – EcCAP (mutant) + cAMP
3i54, 3i59 – MtCAP + cAMP

CAP complex with cAMP and DNA

1cgp, 1run, 2cgp, 1j59, 1o3q, 1o3r, 1o3s, 1o3t, 1zrc, 1zrd, 1zre, 1zrf – EcCAP + cAMP + DNA
1ruo – EcCAP (mutant) + cAMP + DNA

CAP complex with cAMP, DNA and DNA directed polymerase

1lb2, 3n4m – EcCAP + cAMP + DNA + DNA-directed RNA polymerase α subunit
3iyd – EcCAP + cAMP + DNA + DNA-directed RNA polymerase α, β, ω subunits + RNA polymerase σ factor – Cryo EM