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3hck
From Proteopedia
(New page: 200px<br /> <applet load="3hck" size="450" color="white" frame="true" align="right" spinBox="true" caption="3hck" /> '''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK S...) |
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| - | [[Image:3hck.gif|left|200px]]<br /> | + | [[Image:3hck.gif|left|200px]]<br /><applet load="3hck" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="3hck" size=" | + | |
caption="3hck" /> | caption="3hck" /> | ||
'''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES'''<br /> | '''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The hematopoietic cellular kinase (Hck) is a member of the Src family of | + | The hematopoietic cellular kinase (Hck) is a member of the Src family of non-receptor protein-tyrosine kinases and participates in signal transduction events regulating the growth, differentiation and function of phagocytes. The secondary structure of the SH2 domain for Hck was determined for a 13C/15N-enriched sample using multi-dimensional NMR spectroscopy. The secondary structure for the domain was determined from chemical shift indices [1H alpha, 13C alpha and 13C'], sequential NOEs [d(alphaN)(i, i+1) and d(NN)(i, i+1)], and 3J(alphaN) scalar coupling constants. The Hck SH2 domain consists of two alpha-helices and seven beta-strands. Complementary strands of beta-sheets were identified from long-range NOEs using a novel 3D, 13C/15N-edited HMQC-NOESY-(HCACO)NH experiment that correlated 1H alpha resonances between beta-strands. The secondary structure for Hck SH2 is similar to that predicted from the sequence alignment of the Src-family protein tyrosine kinases. |
==About this Structure== | ==About this Structure== | ||
| - | 3HCK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 3HCK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HCK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Gmeiner, W | + | [[Category: Gmeiner, W H.]] |
| - | [[Category: Smithgall, T | + | [[Category: Smithgall, T E.]] |
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: hck]] | [[Category: hck]] | ||
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[[Category: tyrosine kinase]] | [[Category: tyrosine kinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:09:42 2008'' |
Revision as of 17:09, 21 February 2008
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NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES
Overview
The hematopoietic cellular kinase (Hck) is a member of the Src family of non-receptor protein-tyrosine kinases and participates in signal transduction events regulating the growth, differentiation and function of phagocytes. The secondary structure of the SH2 domain for Hck was determined for a 13C/15N-enriched sample using multi-dimensional NMR spectroscopy. The secondary structure for the domain was determined from chemical shift indices [1H alpha, 13C alpha and 13C'], sequential NOEs [d(alphaN)(i, i+1) and d(NN)(i, i+1)], and 3J(alphaN) scalar coupling constants. The Hck SH2 domain consists of two alpha-helices and seven beta-strands. Complementary strands of beta-sheets were identified from long-range NOEs using a novel 3D, 13C/15N-edited HMQC-NOESY-(HCACO)NH experiment that correlated 1H alpha resonances between beta-strands. The secondary structure for Hck SH2 is similar to that predicted from the sequence alignment of the Src-family protein tyrosine kinases.
About this Structure
3HCK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Sequential assignment and secondary structure determination for the Src homology 2 domain of hematopoietic cellular kinase., Zhang W, Smithgall TE, Gmeiner WH, FEBS Lett. 1997 Apr 7;406(1-2):131-5. PMID:9109402
Page seeded by OCA on Thu Feb 21 19:09:42 2008
