3kvt
From Proteopedia
(New page: 200px<br /><applet load="3kvt" size="350" color="white" frame="true" align="right" spinBox="true" caption="3kvt, resolution 2.00Å" /> '''TETRAMERIZATION DOMA...) |
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==Overview== | ==Overview== | ||
| - | The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated | + | The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bellamy, H.]] | [[Category: Bellamy, H.]] | ||
| - | [[Category: Bixby, K | + | [[Category: Bixby, K A.]] |
[[Category: Choe, S.]] | [[Category: Choe, S.]] | ||
[[Category: Kreusch, A.]] | [[Category: Kreusch, A.]] | ||
| - | [[Category: Nanao, M | + | [[Category: Nanao, M H.]] |
| - | [[Category: Pfaffinger, P | + | [[Category: Pfaffinger, P J.]] |
| - | [[Category: Shen, N | + | [[Category: Shen, N V.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: molecular recognition]] | [[Category: molecular recognition]] | ||
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[[Category: zinc-binding]] | [[Category: zinc-binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:10:01 2008'' |
Revision as of 17:10, 21 February 2008
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TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL
Overview
The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.
About this Structure
3KVT is a Single protein structure of sequence from Aplysia californica with as ligand. Full crystallographic information is available from OCA.
Reference
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:9886290
Page seeded by OCA on Thu Feb 21 19:10:01 2008
