3rpb
From Proteopedia
(New page: 200px<br /><applet load="3rpb" size="450" color="white" frame="true" align="right" spinBox="true" caption="3rpb" /> '''THE C2B-DOMAIN OF RABPHILIN: STRUCTURAL VARI...) |
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| - | [[Image:3rpb.gif|left|200px]]<br /><applet load="3rpb" size=" | + | [[Image:3rpb.gif|left|200px]]<br /><applet load="3rpb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="3rpb" /> | caption="3rpb" /> | ||
'''THE C2B-DOMAIN OF RABPHILIN: STRUCTURAL VARIATIONS IN A JANUS-FACED DOMAIN'''<br /> | '''THE C2B-DOMAIN OF RABPHILIN: STRUCTURAL VARIATIONS IN A JANUS-FACED DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | C2 domains are widespread protein modules that often occur as tandem | + | C2 domains are widespread protein modules that often occur as tandem repeats in many membrane-trafficking proteins such as synaptotagmin and rabphilin. The first and second C2 domains (C2A and C2B, respectively) have a high degree of homology but also specific differences. The structure of the C2A domain of synaptotagmin I has been extensively studied but little is known about the C2B domains. We have used NMR spectroscopy to determine the solution structure of the C2B domain of rabphilin. The overall structure of the C2B domain is very similar to that of other C2 domains, with a rigid beta-sandwich core and loops at the top (where Ca2+ binds) and the bottom. Surprisingly, a relatively long alpha-helix is inserted at the bottom of the domain and is conserved in all C2B domains. Our results, together with the Ca(2+)-independent interactions observed for C2B domains, indicate that these domains have a Janus-faced nature, with a Ca(2+)-binding top surface and a Ca(2+)-independent bottom surface. |
==About this Structure== | ==About this Structure== | ||
| - | 3RPB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 3RPB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Garcia, J.]] | [[Category: Garcia, J.]] | ||
| - | [[Category: Nittler, M | + | [[Category: Nittler, M P.]] |
[[Category: Rizo, J.]] | [[Category: Rizo, J.]] | ||
| - | [[Category: Sudhof, T | + | [[Category: Sudhof, T C.]] |
[[Category: Ubach, J.]] | [[Category: Ubach, J.]] | ||
[[Category: c2-domains]] | [[Category: c2-domains]] | ||
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[[Category: rabphilin]] | [[Category: rabphilin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:14 2008'' |
Revision as of 17:11, 21 February 2008
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THE C2B-DOMAIN OF RABPHILIN: STRUCTURAL VARIATIONS IN A JANUS-FACED DOMAIN
Overview
C2 domains are widespread protein modules that often occur as tandem repeats in many membrane-trafficking proteins such as synaptotagmin and rabphilin. The first and second C2 domains (C2A and C2B, respectively) have a high degree of homology but also specific differences. The structure of the C2A domain of synaptotagmin I has been extensively studied but little is known about the C2B domains. We have used NMR spectroscopy to determine the solution structure of the C2B domain of rabphilin. The overall structure of the C2B domain is very similar to that of other C2 domains, with a rigid beta-sandwich core and loops at the top (where Ca2+ binds) and the bottom. Surprisingly, a relatively long alpha-helix is inserted at the bottom of the domain and is conserved in all C2B domains. Our results, together with the Ca(2+)-independent interactions observed for C2B domains, indicate that these domains have a Janus-faced nature, with a Ca(2+)-binding top surface and a Ca(2+)-independent bottom surface.
About this Structure
3RPB is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure of the Janus-faced C2B domain of rabphilin., Ubach J, Garcia J, Nittler MP, Sudhof TC, Rizo J, Nat Cell Biol. 1999 Jun;1(2):106-12. PMID:10559882
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