425d
From Proteopedia
(New page: 200px<br /><applet load="425d" size="350" color="white" frame="true" align="right" spinBox="true" caption="425d, resolution 2.80Å" /> '''5'-D(*AP*CP*CP*GP*GP...) |
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==Overview== | ==Overview== | ||
| - | Transcriptional regulation in papillomaviruses depends on | + | Transcriptional regulation in papillomaviruses depends on sequence-specific binding of the regulatory protein E2 to several sites in the viral genome. Crystal structures of bovine papillomavirus E2 DNA targets reveal a conformational variant of B-DNA characterized by a roll-induced writhe and helical repeat of 10.5 bp per turn. A comparison between the free and the protein-bound DNA demonstrates that the intrinsic structure of the DNA regions contacted directly by the protein and the deformability of the DNA region that is not contacted by the protein are critical for sequence-specific protein/DNA recognition and hence for gene-regulatory signals in the viral system. We show that the selection of dinucleotide or longer segments with appropriate conformational characteristics, when positioned at correct intervals along the DNA helix, can constitute a structural code for DNA recognition by regulatory proteins. This structural code facilitates the formation of a complementary protein-DNA interface that can be further specified by hydrogen bonds and nonpolar interactions between the protein amino acids and the DNA bases. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Frolow, F.]] | [[Category: Frolow, F.]] | ||
| - | [[Category: Hegde, R | + | [[Category: Hegde, R S.]] |
[[Category: Rabinovich, D.]] | [[Category: Rabinovich, D.]] | ||
[[Category: Rozenberg, H.]] | [[Category: Rozenberg, H.]] | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:06 2008'' |
Revision as of 17:12, 21 February 2008
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5'-D(*AP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*T)-3'
Overview
Transcriptional regulation in papillomaviruses depends on sequence-specific binding of the regulatory protein E2 to several sites in the viral genome. Crystal structures of bovine papillomavirus E2 DNA targets reveal a conformational variant of B-DNA characterized by a roll-induced writhe and helical repeat of 10.5 bp per turn. A comparison between the free and the protein-bound DNA demonstrates that the intrinsic structure of the DNA regions contacted directly by the protein and the deformability of the DNA region that is not contacted by the protein are critical for sequence-specific protein/DNA recognition and hence for gene-regulatory signals in the viral system. We show that the selection of dinucleotide or longer segments with appropriate conformational characteristics, when positioned at correct intervals along the DNA helix, can constitute a structural code for DNA recognition by regulatory proteins. This structural code facilitates the formation of a complementary protein-DNA interface that can be further specified by hydrogen bonds and nonpolar interactions between the protein amino acids and the DNA bases.
About this Structure
425D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural code for DNA recognition revealed in crystal structures of papillomavirus E2-DNA targets., Rozenberg H, Rabinovich D, Frolow F, Hegde RS, Shakked Z, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15194-9. PMID:9860945
Page seeded by OCA on Thu Feb 21 19:12:06 2008
