437d
From Proteopedia
(New page: 200px<br /><applet load="437d" size="350" color="white" frame="true" align="right" spinBox="true" caption="437d, resolution 1.6Å" /> '''CRYSTAL STRUCTURE OF ...) |
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==Overview== | ==Overview== | ||
| - | Many viruses regulate translation of polycistronic mRNA using a -1 | + | Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting. |
==About this Structure== | ==About this Structure== | ||
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Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074948 10074948] | Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074948 10074948] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
[[Category: Chen, L.]] | [[Category: Chen, L.]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
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[[Category: viral frameshifting]] | [[Category: viral frameshifting]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:11 2008'' |
Revision as of 17:12, 21 February 2008
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CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING
Overview
Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.
About this Structure
437D is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948
Page seeded by OCA on Thu Feb 21 19:12:11 2008
Categories: Protein complex | Berger, J M. | Chen, L. | Egli, M. | Rich, A. | Su, L. | MG | NA | Pseudoknot | Rna | Triplex | Viral frameshifting
