4aig
From Proteopedia
(New page: 200px<br /><applet load="4aig" size="450" color="white" frame="true" align="right" spinBox="true" caption="4aig, resolution 2.00Å" /> '''ADAMALYSIN II WITH P...) |
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| - | [[Image:4aig.gif|left|200px]]<br /><applet load="4aig" size=" | + | [[Image:4aig.gif|left|200px]]<br /><applet load="4aig" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="4aig, resolution 2.00Å" /> | caption="4aig, resolution 2.00Å" /> | ||
'''ADAMALYSIN II WITH PHOSPHONATE INHIBITOR'''<br /> | '''ADAMALYSIN II WITH PHOSPHONATE INHIBITOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The search of reprolysin inhibitors offers the possibility of intervention | + | The search of reprolysin inhibitors offers the possibility of intervention against both matrixins and ADAMs. Here we report the crystal structure of the complex between adamalysin II, a member of the reprolysin family, and a phosphonate inhibitor modeled on an endogenous venom tripeptide. The inhibitor occupies the primed region of the cleavage site adopting a retro-binding mode. The phosphonate group ligates the zinc ion in an asymmetric bidentate mode and the adjacent Trp indole system partly fills the primary specificity subsite S1'. An adamalysin-based model of tumor necrosis factor-alpha-converting enzyme (TACE) reveals a smaller S1' pocket for this enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 4AIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Crotalus_adamanteus Crotalus adamanteus] with ZN, CA and FLX as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adamalysin Adamalysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.46 3.4.24.46] Full crystallographic information is available from [http:// | + | 4AIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Crotalus_adamanteus Crotalus adamanteus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=FLX:'>FLX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adamalysin Adamalysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.46 3.4.24.46] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc protease]] | [[Category: zinc protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:39 2008'' |
Revision as of 17:12, 21 February 2008
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ADAMALYSIN II WITH PHOSPHONATE INHIBITOR
Overview
The search of reprolysin inhibitors offers the possibility of intervention against both matrixins and ADAMs. Here we report the crystal structure of the complex between adamalysin II, a member of the reprolysin family, and a phosphonate inhibitor modeled on an endogenous venom tripeptide. The inhibitor occupies the primed region of the cleavage site adopting a retro-binding mode. The phosphonate group ligates the zinc ion in an asymmetric bidentate mode and the adjacent Trp indole system partly fills the primary specificity subsite S1'. An adamalysin-based model of tumor necrosis factor-alpha-converting enzyme (TACE) reveals a smaller S1' pocket for this enzyme.
About this Structure
4AIG is a Single protein structure of sequence from Crotalus adamanteus with , and as ligands. Active as Adamalysin, with EC number 3.4.24.46 Full crystallographic information is available from OCA.
Reference
2 angstrom X-ray structure of adamalysin II complexed with a peptide phosphonate inhibitor adopting a retro-binding mode., Cirilli M, Gallina C, Gavuzzo E, Giordano C, Gomis-Ruth FX, Gorini B, Kress LF, Mazza F, Paradisi MP, Pochetti G, Politi V, FEBS Lett. 1997 Dec 1;418(3):319-22. PMID:9428736
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