4gcr
From Proteopedia
(New page: 200px<br /><applet load="4gcr" size="450" color="white" frame="true" align="right" spinBox="true" caption="4gcr, resolution 1.47Å" /> '''STRUCTURE OF THE BOV...) |
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| - | [[Image:4gcr.jpg|left|200px]]<br /><applet load="4gcr" size=" | + | [[Image:4gcr.jpg|left|200px]]<br /><applet load="4gcr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="4gcr, resolution 1.47Å" /> | caption="4gcr, resolution 1.47Å" /> | ||
'''STRUCTURE OF THE BOVINE EYE LENS PROTEIN GAMMA-B (GAMMA-II)-CRYSTALLIN AT 1.47 ANGSTROMS'''<br /> | '''STRUCTURE OF THE BOVINE EYE LENS PROTEIN GAMMA-B (GAMMA-II)-CRYSTALLIN AT 1.47 ANGSTROMS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The molecular structure of calf gammaB-crystallin (previously called | + | The molecular structure of calf gammaB-crystallin (previously called gammaII), a lens-specific protein, has been refined to a crystallographic R factor of 18.1% for all reflection data, between 8.0 and 1.47 A, 25 959 hkl measured at 293 (1) K. 230 water molecules have been defined by difference Fourier techniques and included in a restrained least-squares refinement. Difference Fourier maps clearly indicated the presence of multiple sites for the sulfur atoms of Cys 18 and Cys 22 which were therefore given coupled second-site occupancies during the refinement. The sulfur atom in the major position of Cys 22 is in the reduced state. Either of the Cys 18 sites can form a high-energy disulfide bridge with the minor position of Cys 22. The position of the carboxy terminus and many other surface side chains have been further defined including the RGD signal peptide. The hydration of the backbone and the interdomain region has been analysed. 27 water molecules make extensive contacts to a single protein molecule and thus contribute to its stability. |
==About this Structure== | ==About this Structure== | ||
| - | 4GCR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure | + | 4GCR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry 1GCR. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GCR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blundell, T | + | [[Category: Blundell, T L.]] |
| - | [[Category: Driessen, H | + | [[Category: Driessen, H P.C.]] |
[[Category: Lindley, P.]] | [[Category: Lindley, P.]] | ||
| - | [[Category: Moss, D | + | [[Category: Moss, D S.]] |
[[Category: Najmudin, S.]] | [[Category: Najmudin, S.]] | ||
[[Category: Nalini, V.]] | [[Category: Nalini, V.]] | ||
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[[Category: eye lens protein]] | [[Category: eye lens protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:13:22 2008'' |
Revision as of 17:13, 21 February 2008
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STRUCTURE OF THE BOVINE EYE LENS PROTEIN GAMMA-B (GAMMA-II)-CRYSTALLIN AT 1.47 ANGSTROMS
Overview
The molecular structure of calf gammaB-crystallin (previously called gammaII), a lens-specific protein, has been refined to a crystallographic R factor of 18.1% for all reflection data, between 8.0 and 1.47 A, 25 959 hkl measured at 293 (1) K. 230 water molecules have been defined by difference Fourier techniques and included in a restrained least-squares refinement. Difference Fourier maps clearly indicated the presence of multiple sites for the sulfur atoms of Cys 18 and Cys 22 which were therefore given coupled second-site occupancies during the refinement. The sulfur atom in the major position of Cys 22 is in the reduced state. Either of the Cys 18 sites can form a high-energy disulfide bridge with the minor position of Cys 22. The position of the carboxy terminus and many other surface side chains have been further defined including the RGD signal peptide. The hydration of the backbone and the interdomain region has been analysed. 27 water molecules make extensive contacts to a single protein molecule and thus contribute to its stability.
About this Structure
4GCR is a Single protein structure of sequence from Bos taurus. This structure supersedes the now removed PDB entry 1GCR. Full crystallographic information is available from OCA.
Reference
Structure of the bovine eye lens protein gammaB(gammaII)-crystallin at 1.47 A., Najmudin S, Nalini V, Driessen HP, Slingsby C, Blundell TL, Moss DS, Lindley PF, Acta Crystallogr D Biol Crystallogr. 1993 Mar 1;49(Pt 2):223-33. PMID:15299528
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