2m8d
From Proteopedia
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{{STRUCTURE_2m8d| PDB=2m8d | SCENE= }} | {{STRUCTURE_2m8d| PDB=2m8d | SCENE= }} | ||
===Structure of SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3'=== | ===Structure of SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3'=== | ||
| + | {{ABSTRACT_PUBMED_23836656}} | ||
==Function== | ==Function== | ||
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==About this Structure== | ==About this Structure== | ||
| - | [[2m8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | [[2m8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8D OCA]. |
==Reference== | ==Reference== | ||
| - | <references group="xtra"/><references/> | + | <ref group="xtra">PMID:023836656</ref><references group="xtra"/><references/> |
| - | [[Category: | + | [[Category: Human]] |
[[Category: Allain, F H.T.]] | [[Category: Allain, F H.T.]] | ||
[[Category: Anczukow, O.]] | [[Category: Anczukow, O.]] | ||
Revision as of 13:46, 12 March 2014
Contents |
Structure of SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3'
Template:ABSTRACT PUBMED 23836656
Function
[SRSF1_HUMAN] Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.[1] [2]
About this Structure
2m8d is a 2 chain structure with sequence from Human. Full experimental information is available from OCA.
Reference
- Clery A, Sinha R, Anczukow O, Corrionero A, Moursy A, Daubner GM, Valcarcel J, Krainer AR, Allain FH. Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):E2802-11. doi:, 10.1073/pnas.1303445110. Epub 2013 Jul 8. PMID:23836656 doi:http://dx.doi.org/10.1073/pnas.1303445110
- ↑ Zuo P, Manley JL. Functional domains of the human splicing factor ASF/SF2. EMBO J. 1993 Dec;12(12):4727-37. PMID:8223481
- ↑ Kohtz JD, Jamison SF, Will CL, Zuo P, Luhrmann R, Garcia-Blanco MA, Manley JL. Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors. Nature. 1994 Mar 10;368(6467):119-24. PMID:8139654 doi:http://dx.doi.org/10.1038/368119a0
