4rnp
From Proteopedia
(New page: 200px<br /><applet load="4rnp" size="450" color="white" frame="true" align="right" spinBox="true" caption="4rnp, resolution 3.0Å" /> '''BACTERIOPHAGE T7 RNA ...) |
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| - | [[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size=" | + | [[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="4rnp, resolution 3.0Å" /> | caption="4rnp, resolution 3.0Å" /> | ||
'''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''<br /> | '''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of T7 RNA polymerase reveals a molecule organized | + | The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined. |
==About this Structure== | ==About this Structure== | ||
| - | 4RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure | + | 4RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure supersedes the now removed PDB entries 3RNP and 2RNP. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Liu, Z | + | [[Category: Liu, Z J.]] |
| - | [[Category: Rose, J | + | [[Category: Rose, J P.]] |
[[Category: Sousa, R.]] | [[Category: Sousa, R.]] | ||
| - | [[Category: Wang, B | + | [[Category: Wang, B C.]] |
[[Category: nucleotidyltransferase]] | [[Category: nucleotidyltransferase]] | ||
[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:14:15 2008'' |
Revision as of 17:14, 21 February 2008
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BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY
Overview
The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.
About this Structure
4RNP is a Single protein structure of sequence from Bacteriophage t7. This structure supersedes the now removed PDB entries 3RNP and 2RNP. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution., Sousa R, Chung YJ, Rose JP, Wang BC, Nature. 1993 Aug 12;364(6438):593-9. PMID:7688864
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