5eas
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have | + | Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have been a source of these natural products by providing a homologous set of terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with two farnesyl diphosphate analogs were analyzed. These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes. As such, these structures provide templates for the engineering of novel terpene cyclases. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Back, K.]] | [[Category: Back, K.]] | ||
[[Category: Chappell, J.]] | [[Category: Chappell, J.]] | ||
| - | [[Category: Noel, J | + | [[Category: Noel, J P.]] |
| - | [[Category: Starks, C | + | [[Category: Starks, C M.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: 5-epi-aristolochene synthase]] | [[Category: 5-epi-aristolochene synthase]] | ||
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[[Category: natural products biosynthesis]] | [[Category: natural products biosynthesis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:15:05 2008'' |
Revision as of 17:15, 21 February 2008
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5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM
Overview
Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have been a source of these natural products by providing a homologous set of terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with two farnesyl diphosphate analogs were analyzed. These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes. As such, these structures provide templates for the engineering of novel terpene cyclases.
About this Structure
5EAS is a Single protein structure of sequence from Nicotiana tabacum with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase., Starks CM, Back K, Chappell J, Noel JP, Science. 1997 Sep 19;277(5333):1815-20. PMID:9295271
Page seeded by OCA on Thu Feb 21 19:15:05 2008
