5gst
From Proteopedia
(New page: 200px<br /><applet load="5gst" size="450" color="white" frame="true" align="right" spinBox="true" caption="5gst, resolution 2.0Å" /> '''REACTION COORDINATE M...) |
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| - | [[Image:5gst.gif|left|200px]]<br /><applet load="5gst" size=" | + | [[Image:5gst.gif|left|200px]]<br /><applet load="5gst" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="5gst, resolution 2.0Å" /> | caption="5gst, resolution 2.0Å" /> | ||
'''REACTION COORDINATE MOTION IN AN SNAR REACTION CATALYZED BY GLUTATHIONE TRANSFERASE'''<br /> | '''REACTION COORDINATE MOTION IN AN SNAR REACTION CATALYZED BY GLUTATHIONE TRANSFERASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structures of a class mu glutathione transferase in | + | The three-dimensional structures of a class mu glutathione transferase in complex with a transition-state analogue, 1-(S-glutathionyl)-2,4,6-trinitrocyclohexadienate, and a product, 1-(S-glutathionyl)-2,4-dinitrobenzene, of a nucleophilic aromatic substitution (SNAr) reaction have been determined at 1.9- and 2.0-A resolution, respectively. The two structures represent snapshots along the reaction coordinate for the enzyme-catalyzed reaction of glutathione with 1-chloro-2,4-dinitrobenzene and reveal specific interactions between the enzyme, intermediate, and product that are important in catalysis. The geometries of the intermediate and product are used to postulate reaction coordinate motion during catalysis. |
==About this Structure== | ==About this Structure== | ||
| - | 5GST is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with SO4 and GDN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http:// | + | 5GST is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GDN:'>GDN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GST OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus rattus]] | [[Category: Rattus rattus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Armstrong, R | + | [[Category: Armstrong, R N.]] |
| - | [[Category: Gilliland, G | + | [[Category: Gilliland, G L.]] |
[[Category: Ji, X.]] | [[Category: Ji, X.]] | ||
[[Category: GDN]] | [[Category: GDN]] | ||
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[[Category: glutathione transferase]] | [[Category: glutathione transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:15:20 2008'' |
Revision as of 17:15, 21 February 2008
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REACTION COORDINATE MOTION IN AN SNAR REACTION CATALYZED BY GLUTATHIONE TRANSFERASE
Overview
The three-dimensional structures of a class mu glutathione transferase in complex with a transition-state analogue, 1-(S-glutathionyl)-2,4,6-trinitrocyclohexadienate, and a product, 1-(S-glutathionyl)-2,4-dinitrobenzene, of a nucleophilic aromatic substitution (SNAr) reaction have been determined at 1.9- and 2.0-A resolution, respectively. The two structures represent snapshots along the reaction coordinate for the enzyme-catalyzed reaction of glutathione with 1-chloro-2,4-dinitrobenzene and reveal specific interactions between the enzyme, intermediate, and product that are important in catalysis. The geometries of the intermediate and product are used to postulate reaction coordinate motion during catalysis.
About this Structure
5GST is a Single protein structure of sequence from Rattus rattus with and as ligands. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
Snapshots along the reaction coordinate of an SNAr reaction catalyzed by glutathione transferase., Ji X, Armstrong RN, Gilliland GL, Biochemistry. 1993 Dec 7;32(48):12949-54. PMID:8241147
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