6cgt
From Proteopedia
(New page: 200px<br /><applet load="6cgt" size="450" color="white" frame="true" align="right" spinBox="true" caption="6cgt, resolution 2.6Å" /> '''HOXA COMPLEX OF CYCLO...) |
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| - | [[Image:6cgt.gif|left|200px]]<br /><applet load="6cgt" size=" | + | [[Image:6cgt.gif|left|200px]]<br /><applet load="6cgt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="6cgt, resolution 2.6Å" /> | caption="6cgt, resolution 2.6Å" /> | ||
'''HOXA COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT'''<br /> | '''HOXA COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic | + | Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds. |
==About this Structure== | ==About this Structure== | ||
| - | 6CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http:// | + | 6CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CGT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Parsiegla, G.]] | [[Category: Parsiegla, G.]] | ||
| - | [[Category: Schulz, G | + | [[Category: Schulz, G E.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: cyclodextrin]] | [[Category: cyclodextrin]] | ||
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[[Category: starch degradation]] | [[Category: starch degradation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:07 2008'' |
Revision as of 17:16, 21 February 2008
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HOXA COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT
Overview
Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds.
About this Structure
6CGT is a Single protein structure of sequence from Bacillus circulans with as ligand. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.
Reference
Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production., Parsiegla G, Schmidt AK, Schulz GE, Eur J Biochem. 1998 Aug 1;255(3):710-7. PMID:9738912
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