6ebx
From Proteopedia
(New page: 200px<br /><applet load="6ebx" size="450" color="white" frame="true" align="right" spinBox="true" caption="6ebx, resolution 1.7Å" /> '''STRUCTURE DETERMINATI...) |
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| - | [[Image:6ebx.gif|left|200px]]<br /><applet load="6ebx" size=" | + | [[Image:6ebx.gif|left|200px]]<br /><applet load="6ebx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="6ebx, resolution 1.7Å" /> | caption="6ebx, resolution 1.7Å" /> | ||
'''STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION'''<br /> | '''STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four | + | Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four disulfide bridges, was crystallized in a new orthorhombic form by using thiocyanate as crystallizing agent. The space group is P2(1)2(1)2(1) with a = 53.36 (4), b = 40.89 (4), c = 55.71 (5) A, V = 121533.1 A and Z = 8. X-ray diffraction data were recorded at the LURE synchrotron facility (lambda = 1.405 A). The structure was solved by molecular replacement and shows a dimeric association through an anti-parallel beta-sheet around the twofold non-crystallographic axis. The two independent molecules, one SCN- ion and 97 associated water molecules were refined by molecular dynamics and annealing techniques to R = 19.6% (10,913 Fobs, resolution 5-1.7 A). The thiocyanate ion is located at the interface of the dimer and close to the non-crystallographic twofold axis. |
==About this Structure== | ==About this Structure== | ||
| - | 6EBX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata] with SCN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 6EBX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata] with <scene name='pdbligand=SCN:'>SCN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:14 2008'' |
Revision as of 17:16, 21 February 2008
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STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION
Overview
Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four disulfide bridges, was crystallized in a new orthorhombic form by using thiocyanate as crystallizing agent. The space group is P2(1)2(1)2(1) with a = 53.36 (4), b = 40.89 (4), c = 55.71 (5) A, V = 121533.1 A and Z = 8. X-ray diffraction data were recorded at the LURE synchrotron facility (lambda = 1.405 A). The structure was solved by molecular replacement and shows a dimeric association through an anti-parallel beta-sheet around the twofold non-crystallographic axis. The two independent molecules, one SCN- ion and 97 associated water molecules were refined by molecular dynamics and annealing techniques to R = 19.6% (10,913 Fobs, resolution 5-1.7 A). The thiocyanate ion is located at the interface of the dimer and close to the non-crystallographic twofold axis.
About this Structure
6EBX is a Single protein structure of sequence from Laticauda semifasciata with as ligand. Full crystallographic information is available from OCA.
Reference
Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution., Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A, Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:1418823
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