Journal:JBIC:24

Solution structure and metal ion binding sites of the human CPEB3 ribozyme's P4 domain

Miriam Skilandat, Magdalena Rowinska-Zyrek and Roland K. O. Sigel ^[1]

Molecular Tour
The structure of the P4 hairpin provides the first structural information on the solution structure of the CPEB3 ribozyme, a highly conserved self-cleaving RNA sequence encoded in the genome of humans and other mammals. CPEB3 is one of very few examples of small ribozymes occuring in human and the only ribozyme that is suggested to be of human origin. Its function, however, is not yet understood. The P4 hairpin points out of the complex interlaced structure of the CPEB3 ribozyme, it consists of a helix adopting the regular fold typical for RNA duplexes and is capped by a UGGU tetraloop.

The secondary structure of the CPEB3 ribozyme and the binding sites of Mg2+ and hexammine(III) cobalt that is an NMR-detectable mimic of a hexahydrated Mg2+ ion. P4 hairpin structure is shown with the two suggested metal-ion binding sites.

(see also image above) ions giving an excellent example of the two different binding modes of Mg(II) to RNA, which are direct coordination and coordination via a shell of water molecules. One of these binding sites probably confers extra stability to the fold of the tetraloop while the other one may play a role in stabilizing the adjacent active site region of the CPEB3 ribozyme. The structure of the loop nucleotides is in this region. within this new tetraloop structure and a putative hydrogen bond stabilize the hairpin fold which is similar to the AGUU tetraloop structure serving as a protein-binding motif in another RNA. Due to its exposed position and stable fold P4 could serve as an interaction site of the CPEB3 ribozyme for other biomolecules involved in regulating ribozymatic activity.