6msf
From Proteopedia
(New page: 200px<br /><applet load="6msf" size="450" color="white" frame="true" align="right" spinBox="true" caption="6msf, resolution 2.800Å" /> '''F6 APTAMER MS2 COAT...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:6msf.gif|left|200px]]<br /><applet load="6msf" size=" | + | [[Image:6msf.gif|left|200px]]<br /><applet load="6msf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="6msf, resolution 2.800Å" /> | caption="6msf, resolution 2.800Å" /> | ||
'''F6 APTAMER MS2 COAT PROTEIN COMPLEX'''<br /> | '''F6 APTAMER MS2 COAT PROTEIN COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to | + | The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to bacteriophage MS2 coat protein has been determined. It provides an opportunity to compare the interactions of MS2 coat protein and wild type operator with those of an aptamer, whose secondary structure differs from the wild type RNA in having a three-base loop (compared to a tetraloop) and an additional base pair between this loop and the sequence-specific recognition element in the stem. The RNA binds in the same location on the coat protein as the wild type operator and maintains many of the same RNA-protein interactions. In order to achieve this, the RNA stem loop undergoes a concerted rearrangement of the 3' side while leaving the 5' side and the loop interactions largely unchanged, illustrating the ability of RNA to present similar molecular recognition surfaces from distinct primary and secondary structures. |
==About this Structure== | ==About this Structure== | ||
| - | 6MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http:// | + | 6MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MSF OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Enterobacterio phage ms2]] | [[Category: Enterobacterio phage ms2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Convery, M | + | [[Category: Convery, M A.]] |
| - | [[Category: Ellington, A | + | [[Category: Ellington, A D.]] |
[[Category: Hirao, I.]] | [[Category: Hirao, I.]] | ||
| - | [[Category: Murray, J | + | [[Category: Murray, J B.]] |
| - | [[Category: Peabody, D | + | [[Category: Peabody, D S.]] |
| - | [[Category: Phillips, S | + | [[Category: Phillips, S E.V.]] |
[[Category: Rowsell, S.]] | [[Category: Rowsell, S.]] | ||
| - | [[Category: Stockley, P | + | [[Category: Stockley, P G.]] |
| - | [[Category: Stonehouse, N | + | [[Category: Stonehouse, N J.]] |
[[Category: bacteriophage ms2]] | [[Category: bacteriophage ms2]] | ||
[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
| Line 28: | Line 28: | ||
[[Category: viral coat protein/rna complex]] | [[Category: viral coat protein/rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:32 2008'' |
Revision as of 17:16, 21 February 2008
|
F6 APTAMER MS2 COAT PROTEIN COMPLEX
Overview
The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to bacteriophage MS2 coat protein has been determined. It provides an opportunity to compare the interactions of MS2 coat protein and wild type operator with those of an aptamer, whose secondary structure differs from the wild type RNA in having a three-base loop (compared to a tetraloop) and an additional base pair between this loop and the sequence-specific recognition element in the stem. The RNA binds in the same location on the coat protein as the wild type operator and maintains many of the same RNA-protein interactions. In order to achieve this, the RNA stem loop undergoes a concerted rearrangement of the 3' side while leaving the 5' side and the loop interactions largely unchanged, illustrating the ability of RNA to present similar molecular recognition surfaces from distinct primary and secondary structures.
About this Structure
6MSF is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
Reference
Crystal structure of an RNA aptamer-protein complex at 2.8 A resolution., Convery MA, Rowsell S, Stonehouse NJ, Ellington AD, Hirao I, Murray JB, Peabody DS, Phillips SE, Stockley PG, Nat Struct Biol. 1998 Feb;5(2):133-9. PMID:9461079
Page seeded by OCA on Thu Feb 21 19:16:32 2008
Categories: Enterobacterio phage ms2 | Single protein | Convery, M A. | Ellington, A D. | Hirao, I. | Murray, J B. | Peabody, D S. | Phillips, S E.V. | Rowsell, S. | Stockley, P G. | Stonehouse, N J. | Bacteriophage ms2 | Icosahedral virus | Rna aptamer | Rna stem loop | Viral coat protein/rna complex
